ArticleThe New phytologist2009
Sterol metabolism in the oomycete Aphanomyces euteiches, a legume root pathogen.
Article in The New phytologist, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Loss of Sterol Biosynthesis in Economically Important Plant Pests and Pathogens: A Review of a Potential Target for Pest Control.Biomolecules · 2024Review
- Sterol-Sensing Domain (SSD)-Containing Proteins in Sterol AuxotrophicMicrobiology spectrum · 2023Article
- Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins ofJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Functional Analysis of the C-5 Sterol Desaturase PcErg3 in the Sterol Auxotrophic Oomycete PathogenFrontiers in microbiology · 2022Article
- Ecological Dynamics and Microbial Treatments against Oomycete Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
- Advances in steroidal saponins biosynthesis.Planta · 2021Review
- A Review ofPlants (Basel, Switzerland) · 2021Review
- The mysterious route of sterols in oomycetes.PLoS pathogens · 2021Review
- The Mevalonate Pathway Is Important for Growth, Spore Production, and the Virulence ofFrontiers in microbiology · 2021Article
- A Patched-Like Protein PsPTL Is Not Essential for the Growth and Response to Various Stresses inFrontiers in microbiology · 2021Article
- Evolution of the Sterol Biosynthetic Pathway of Pythium insidiosum and Related Oomycetes Contributes to Antifungal Drug Resistance.Antimicrobial agents and chemotherapy · 2017Article
- Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans.PloS one · 2017Article
- The Oxidosqualene Cyclase from the OomyceteFrontiers in microbiology · 2016Article
- Diversity and evolution of cytochrome P450 monooxygenases in Oomycetes.Scientific reports · 2015Article
- The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.PloS one · 2015Article
- The secreted proteins of Achlya hypogyna and Thraustotheca clavata identify the ancestral oomycete secretome and reveal gene acquisitions by horizontal gene transfer.Genome biology and evolution · 2014Article
- Clotrimazole as a potent agent for treating the oomycete fish pathogen Saprolegnia parasitica through inhibition of sterol 14α-demethylase (CYP51).Applied and environmental microbiology · 2014Article
- Distinctive expansion of potential virulence genes in the genome of the oomycete fish pathogen Saprolegnia parasitica.PLoS genetics · 2013Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sterols are isoprenoid-derived molecules that have essential functions in eukaryotes but whose metabolism remains largely unknown in a large number of organisms. Oomycetes are fungus-like microorganisms that are evolutionarily related to stramenopile algae, a large group of organisms for which no sterol metabolic pathway has been reported. Here, we present data that support a model of sterol biosynthesis in Aphanomyces euteiches, an oomycete species causing devastating diseases in legume crops. In silico analyses were performed to identify genes encoding enzymes involved in the conversion of the isoprenoid precursor 3-hydroxy-3-methylglutaryl coenzyme A to isoprenoids. Several metabolic intermediates and two major sterol end-products were identified by gas chromatography-mass spectroscopy. We show that A. euteiches is able to produce fucosterol (a sterol initially identified in brown algae) and cholesterol (the major animal sterol). Mycelium development is inhibited by two sterol demethylase inhibitors used as fungicides, namely tebuconazole and epoxiconazole. We propose the first sterol biosynthetic pathway identified in a stramenopile species. Phylogenetic analyses revealed close relationships between A. euteiches enzyme sequences and those found in stramenopile algae, suggesting that part of this pathway could be conserved in the Stramenopila kingdom.
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