ArticlePlant molecular biology2009
Virus-induced gene silencing in soybean seeds and the emergence stage of soybean plants with Apple latent spherical virus vectors.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 121 citations in OpenAlex.
- Virus-Mediated Overexpression of Two Allelic Protein Fragments Elicits Drastically Different Responses in Soybean.Viruses · 2026Article
- Virus-induced gene silencing inPlant biotechnology (Tokyo, Japan) · 2026Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.Biotechnology journal · 2024Article
- Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System.Plants (Basel, Switzerland) · 2024Article
- The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci.International journal of molecular sciences · 2024Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean.Plant methods · 2022Article
- Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin.Plant methods · 2021Article
- Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing.BMC plant biology · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa.Frontiers in plant science · 2021Article
- Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release.Communications biology · 2020Article
- Soy and Arabidopsis receptor-like kinases respond to polysaccharide signals from Spodoptera species and mediate herbivore resistance.Communications biology · 2020Article
- Estimation of the functions of viral RNA silencing suppressors by apple latent spherical virus vector.Virus genes · 2020Article
- Virus-Induced Flowering by Apple Latent Spherical Virus Vector: Effective Use to Accelerate Breeding of Grapevine.Viruses · 2020Article
- Functional divergence between soybean FLOWERING LOCUS T orthologues FT2a and FT5a in post-flowering stem growth.Journal of experimental botany · 2019Article
- Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands.BMC plant biology · 2019Article
- Accelerating Soybean Breeding in a CO2-Supplemented Growth Chamber.Plant & cell physiology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-induced gene silencing (VIGS) has great potential as a reverse-genetics tool in plant genomics. In this study, we examined the potential of VIGS in soybean seeds and the emergence stage of soybean plants using Apple latent spherical virus (ALSV) vectors. Inoculation of an ALSV vector (soyPDS-ALSV) carrying a fragment of the soybean phytoene desaturase (soyPDS) gene into soybean seedlings resulted in a highly uniform photo-bleached phenotype, typical of PDS inhibition, on the upper leaves throughout plant growth. The photo-bleached phenotype was also found on all immature pods, all seed coats, and about 50% embryos of seeds on soybean plants infected with soyPDS-ALSV. Infection with an ALSV vector (soyIFS2-ALSV) having a fragment of soybean isoflavone synthase 2 (soyIFS2) gene also led to a reduction of the levels of both soyIFS2- and soyIFS1- mRNAs and an isoflavone content in the cotyledons of about 36% mature seeds of infected soybean plants. Furthermore, VIGS of soyPDS was induced in the next generation plants by the seed transmission of soyPDS-ALSV. Thus ALSV vectors will be useful for studying gene functions in the reproductive stages and early growth stages, such as emergence and cotyledon stages, in addition to the vegetative stages of soybean plants.
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