ArticleThe EMBO journal2009
The fungal RNA-binding protein Rrm4 mediates long-distance transport of ubi1 and rho3 mRNAs.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 98 citations in OpenAlex.
- Endosome motility controls light-responsive reproductive development and secondary metabolite production in Aspergillus.Current biology : CB · 2026Article
- Active transport of tRNAs facilitates distributed protein synthesis.bioRxiv : the preprint server for biology · 2026Article
- Vesicle-coupled mRNA transport and translation govern intracellular organelle networking.EMBO reports · 2026Review
- The RRM domain-containing protein Rbp3 interacts with ribosomes and the 3' ends of mRNAs encoding photosynthesis proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Microbial iCLIP2: enhanced mapping of RNA-protein interaction by promoting protein and RNA stability.RNA (New York, N.Y.) · 2025Article
- The mRNA stability factor Khd4 defines a specific mRNA regulon for membrane trafficking in the pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Research progress of live-cell RNA imaging techniques.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Linking transport and translation of mRNAs with endosomes and mitochondria.EMBO reports · 2021Review
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- RNA contributions to the form and function of biomolecular condensates.Nature reviews. Molecular cell biology · 2021Review
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- Versatile CRISPR/Cas9 Systems for Genome Editing inJournal of fungi (Basel, Switzerland) · 2021Article
- Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes.Microorganisms · 2020Review
- Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous FungusFrontiers in microbiology · 2020Article
- Establishing Polycistronic Expression in the Model MicroorganismFrontiers in microbiology · 2020Article
- Complex Interactions Between Membrane-Bound Organelles, Biomolecular Condensates and the Cytoskeleton.Frontiers in cell and developmental biology · 2020Review
- The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.EMBO reports · 2019Article
- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2019Article
- The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs.EMBO reports · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoskeletal transport promotes polar growth in filamentous fungi. In Ustilago maydis, the RNA-binding protein Rrm4 shuttles along microtubules and is crucial for polarity in infectious filaments. Mutations in the RNA-binding domain cause loss of function. However, it was unclear which RNAs are bound and transported. Here, we applied in vivo RNA binding studies and live imaging to determine the molecular function of Rrm4. This new combination revealed that Rrm4 mediates microtubule-dependent transport of distinct mRNAs encoding, for example, the ubiquitin fusion protein Ubi1 and the small G protein Rho3. These transcripts accumulate in ribonucleoprotein particles (mRNPs) that move bidirectionally along microtubules and co-localise with Rrm4. Importantly, the 3' untranslated region of ubi1 containing a CA-rich binding site functions as zipcode during mRNA transport. Furthermore, motile mRNPs are not formed when the RNA-binding domain of Rrm4 is deleted, although the protein is still shuttling. Thus, Rrm4 constitutes an integral component of the transport machinery. We propose that microtubule-dependent mRNP trafficking is crucial for hyphal growth introducing U. maydis as attractive model for studying mRNA transport in higher eukaryotes.
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