ArticleNucleic acids research2009
Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 29 citations in OpenAlex.
- TOP1 and TOP2 complementarily maintain DNA replication fork progression in vertebrates.The Journal of biological chemistry · 2026Article
- DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function.eLife · 2022Article
- Nonlinear mechanics of human mitotic chromosomes.Nature · 2022Article
- Topoisomerase IIα is essential for maintenance of mitotic chromosome structure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Mutual regulation of MDM4 and TOP2A in cancer cell proliferation.Molecular oncology · 2019Article
- A tri-serine cluster within the topoisomerase IIα-interaction domain of the BLM helicase is required for regulating chromosome breakage in human cells.Human molecular genetics · 2018Article
- Site-Specific Cleavage by Topoisomerase 2: A Mark of the Core Centromere.International journal of molecular sciences · 2018Article
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- Non-Catalytic Roles of the Topoisomerase IIα C-Terminal Domain.International journal of molecular sciences · 2017Review
- ATR inhibitors as a synthetic lethal therapy for tumours deficient in ARID1A.Nature communications · 2016Article
- PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis.Nature communications · 2015Article
- TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells.The Journal of cell biology · 2015Article
- Review
- TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.Nature communications · 2015Article
- The analysis of mutant alleles of different strength reveals multiple functions of topoisomerase 2 in regulation of Drosophila chromosome structure.PLoS genetics · 2014Article
- Article
- The α isoform of topoisomerase II is required for hypercompaction of mitotic chromosomes in human cells.Nucleic acids research · 2014Article
- TopBP1/Dpb11 binds DNA anaphase bridges to prevent genome instability.The Journal of cell biology · 2014Article
- Article
- Repair of DNA strand breaks in a minichromosome in vivo: kinetics, modeling, and effects of inhibitors.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
DT40 is a B-cell lymphoma-derived avian cell line widely used to study cell autonomous gene function because of the high rates with which DNA constructs are homologously recombined into its genome. Here, we demonstrate that the power of the DT40 system can be extended yet further through the use of RNA interference as an alternative to gene targeting. We have generated and characterized stable DT40 transfectants in which both topo 2 genes have been in situ tagged using gene targeting, and from which the mRNA of both topoisomerase 2 isoforms can be conditionally depleted through the tetracycline-induced expression of short hairpin RNAs. The cell cycle phenotype of topo 2-depleted DT40 cells has been compared with that previously reported for other vertebrate cells depleted either of topo 2alpha through gene targeting, or depleted of both isoforms simultaneously by transient RNAi. In addition, the DT40 knockdown system has been used to explore whether excess catenation arising through topo 2 depletion is sufficient to trigger the G2 catenation (or decatenation) checkpoint, proposed to exist in differentiated vertebrate cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.