ArticleThe Journal of biological chemistry2009
The roles of multiple importins for nuclear import of murine aristaless-related homeobox protein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
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- The ALX4 dimer structure provides insight into how disease alleles impact function.Nature communications · 2025Article
- Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.Cell death & disease · 2023Article
- Karyopherin-mediated nucleocytoplasmic transport.Nature reviews. Molecular cell biology · 2022Review
- Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.Nature communications · 2021Article
- FEN1 Blockade for Platinum Chemo-Sensitization and Synthetic Lethality in Epithelial Ovarian Cancers.Cancers · 2021Article
- Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein.Bioscience reports · 2020Article
- Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin.BioMed research international · 2015Article
- Autosomal recessive mutations in nuclear transport factor KPNA7 are associated with infantile spasms and cerebellar malformation.European journal of human genetics : EJHG · 2014Article
- Stimulated nuclear import by β-like importins.F1000prime reports · 2013Review
- Nuclear import of aristaless-related homeobox protein via its NLS1 regulates its transcriptional function.Molecular and cellular biochemistry · 2013Article
- A novel role of proteasomal β1 subunit in tumorigenesis.Bioscience reports · 2013Article
- Expression and subcellular distribution of imp13 are regulated in brain development.In vitro cellular & developmental biology. Animal · 2013Article
- Interneuron, interrupted: molecular pathogenesis of ARX mutations and X-linked infantile spasms.Current opinion in neurobiology · 2012Review
- Distinct DNA binding and transcriptional repression characteristics related to different ARX mutations.Neurogenetics · 2012Article
- Nuclear import by karyopherin-βs: recognition and inhibition.Biochimica et biophysica acta · 2011Review
- Karyopherins in nuclear transport of homeodomain proteins during development.Biochimica et biophysica acta · 2011Review
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11 authors at 4 institutions in 2 countries.
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Abstract
Nuclear import of proteins with nuclear localization signals (NLSs) is mediated by shuttling carriers, the importins. Some cargoes display more than a single NLS, and among these are homeodomain proteins such as Arx, which is critical for development of multiple tissues. Arx has two functional NLSs. The present studies show that several pathways can import Arx via its NLS2, which is within its DNA binding homeodomain. Using an in vitro nuclear import assay, we show that import of Arx via NLS2 can be mediated by importin beta1, importin 9, or importin 13, with binding being strongest to importin beta1. All binding is sensitive to RanGTP. Experiments based on precise domain deletions indicate that NLS2 binds impbeta1, imp9, and imp13 and includes both an importin binding subdomain and a regulatory subdomain with arginine residues being important for function. Moreover, Arx can be co-precipitated with these importins when NLS2 is present. Although nuclear import of Arx can be mediated by these three importin betas, importin beta1 seems to play the major role judging from in vivo small interfering RNA ablations and the in vitro import assay. This is the first evidence to show the role of importin beta1 in nuclear import of paired-type homeodomain proteins. We propose a novel and possibly quite general mechanism for nuclear import of paired-type homeodomain proteins which is critical for development.
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