ArticleJournal of clinical microbiology2009
Molecular subtyping of Treponema pallidum subsp. pallidum in Lisbon, Portugal.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 43 citations in OpenAlex.
- Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis.Journal of clinical microbiology · 2015Pooled it
- Molecular typing of Treponema pallidum: a systematic review and meta-analysis.PLoS neglected tropical diseases · 2011Pooled it
- Article
- Detection of Treponema pallidum DNA During Early Syphilis Stages in Peripheral Blood, Oropharynx, Ano-Rectum and Urine as a Proxy for Transmissibility.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Molecular and Direct Detection Tests for Treponema pallidum Subspecies pallidum: A Review of the Literature, 1964-2017.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Review
- Gene subtype analysis ofExperimental and therapeutic medicine · 2018Article
- Development of a Multilocus Sequence Typing (MLST) scheme for Treponema pallidum subsp. pertenue: Application to yaws in Lihir Island, Papua New Guinea.PLoS neglected tropical diseases · 2017Article
- Characterizing the syphilis epidemic among men who have sex with men in Lima, Peru to identify new treatment and control strategies.BMC infectious diseases · 2013Article
- Comparison of CDC and sequence-based molecular typing of syphilis treponemes: tpr and arp loci are variable in multiple samples from the same patient.BMC microbiology · 2013Article
- Molecular typing of Treponema pallidum: a 5-year surveillance in Shanghai, China.Journal of clinical microbiology · 2012Article
- Evaluation of macrolide resistance and enhanced molecular typing of Treponema pallidum in patients with syphilis in Taiwan: a prospective multicenter study.Journal of clinical microbiology · 2012Article
- Molecular subtyping of Treponema pallidum during a local syphilis epidemic in men who have sex with men in Melbourne, Australia.Journal of clinical microbiology · 2012Article
- Evaluation of a PCR test for detection of treponema pallidum in swabs and blood.Journal of clinical microbiology · 2012Article
- Syphilis: using modern approaches to understand an old disease.The Journal of clinical investigation · 2011Review
- Enhanced molecular typing of treponema pallidum: geographical distribution of strain types and association with neurosyphilis.The Journal of infectious diseases · 2010Article
- Secondary syphilis in cali, Colombia: new concepts in disease pathogenesis.PLoS neglected tropical diseases · 2010Article
- Canadian Public Health Laboratory Network laboratory guidelines for the use of direct tests to detect syphilis in Canada.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicaleArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this study were to evaluate the reproducibility of a molecular method for the subtyping of Treponema pallidum subsp. pallidum and to discriminate strains of this microorganism from strains from patients with syphilis. We studied 212 specimens from a total of 82 patients with different stages of syphilis (14 primary, 7 secondary and 61 latent syphilis). The specimens were distributed as follows: genital ulcers (n = 9), skin and mucosal lesions (n = 7), blood (n = 82), plasma (n = 82), and ear lobe scrapings (n = 32). The samples were assayed by a PCR technique to amplify a segment of the polymerase gene I (polA). Positive samples were typed on the basis of the analysis of two variable genes, tpr and arp. Sixty-two of the 90 samples positive for polA yielded typeable Treponema pallidum DNA. All skin lesions in which T. pallidum was identified (six of six [100%]) were found to contain enough DNA for typing of the organism. It was also possible to type DNA from 7/9 (77.7%) genital ulcer samples, 13/22 (59.1%) blood samples, 20/32 (62.5%) plasma samples, and 16/21 (76.2%) ear lobe scrapings. The same subtype was identified in all samples from the same patient. Five molecular subtypes (subtypes 10a, 14a, 14c, 14f, and 14g) were identified, with the most frequently found subtype being subtype 14a and the least frequently found subtype being subtype 10a. In conclusion, the subtyping technique used in this study seems to have good reproducibility. To our knowledge, subtype 10a was identified for the first time. Further studies are needed to explain the presence of this subtype in Portugal, namely, its relationship to the Treponema pallidum strains circulating in the African countries where Portuguese is spoken.
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Registered trials
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