ArticleMolecular biology of the cell2009
The exosome associates cotranscriptionally with the nascent pre-mRNP through interactions with heterogeneous nuclear ribonucleoproteins.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay.Genes · 2024Article
- hnRNPM induces translation switch under hypoxia to promote colon cancer development.EBioMedicine · 2019Article
- SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing.Nucleic acids research · 2018Article
- SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma.BMC genomics · 2018Article
- A conserved virus-induced cytoplasmic TRAMP-like complex recruits the exosome to target viral RNA for degradation.Genes & development · 2016Article
- The Genome-Wide Analysis of Carcinoembryonic Antigen Signaling by Colorectal Cancer Cells Using RNA Sequencing.PloS one · 2016Article
- An Interaction between RRP6 and SU(VAR)3-9 Targets RRP6 to Heterochromatin and Contributes to Heterochromatin Maintenance in Drosophila melanogaster.PLoS genetics · 2015Article
- Fail-safe transcription termination: Because one is never enough.RNA biology · 2015Review
- The RNA exosome promotes transcription termination of backtracked RNA polymerase II.Nature structural & molecular biology · 2014Article
- Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS.Molecular biology reports · 2014Article
- Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing.Nucleic acids research · 2014Article
- Brahma regulates a specific trans-splicing event at the mod(mdg4) locus of Drosophila melanogaster.RNA biology · 2014Article
- Article
- The human cap-binding complex is functionally connected to the nuclear RNA exosome.Nature structural & molecular biology · 2013Article
- mRNA quality control goes transcriptional.Biochemical Society transactions · 2013Review
- Genome-wide localization of exosome components to active promoters and chromatin insulators in Drosophila.Nucleic acids research · 2013Article
- Rrp6 is recruited to transcribed genes and accompanies the spliced mRNA to the nuclear pore.RNA (New York, N.Y.) · 2012Article
- Dis3- and exosome subunit-responsive 3' mRNA instability elements.Biochemical and biophysical research communications · 2012Article
- To the pore and through the pore: a story of mRNA export kinetics.Biochimica et biophysica acta · 2012Review
- Co-transcriptional degradation of aberrant pre-mRNA by Xrn2.The EMBO journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic cells have evolved quality control mechanisms to degrade aberrant mRNA molecules and prevent the synthesis of defective proteins that could be deleterious for the cell. The exosome, a protein complex with ribonuclease activity, is a key player in quality control. An early quality checkpoint takes place cotranscriptionally but little is known about the molecular mechanisms by which the exosome is recruited to the transcribed genes. Here we study the core exosome subunit Rrp4 in two insect model systems, Chironomus and Drosophila. We show that a significant fraction of Rrp4 is associated with the nascent pre-mRNPs and that a specific mRNA-binding protein, Hrp59/hnRNP M, interacts in vivo with multiple exosome subunits. Depletion of Hrp59 by RNA interference reduces the levels of Rrp4 at transcription sites, which suggests that Hrp59 is needed for the exosome to stably interact with nascent pre-mRNPs. Our results lead to a revised mechanistic model for cotranscriptional quality control in which the exosome is constantly recruited to newly synthesized RNAs through direct interactions with specific hnRNP proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.