ArticleAmerican journal of physiology. Renal physiology2009
Activation of the nitric oxide-cGMP pathway reduces phasic contractions in neonatal rat bladder strips via protein kinase G.
Article in American journal of physiology. Renal physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Comparative study of the role of nitric oxide in the regulation of food intake across vertebrates.Journal of veterinary science · 2026Review
- Local Injection of Stem Cells Can Be a Potential Strategy to Improve Bladder Dysfunction after Outlet Obstruction in Rats.International journal of molecular sciences · 2024Article
- Focal adhesion kinase activation is involved in contractile stimulation-induced detrusor muscle contraction in mice.European journal of pharmacology · 2023Article
- Soluble guanylate cyclase mediates the relaxation of healthy and inflamed bladder smooth muscle by aqueous nitric oxide.Frontiers in physiology · 2023Article
- Drugs Currently Undergoing Preclinical or Clinical Trials for the Treatment of Overactive Bladder: A Review.Current therapeutic research, clinical and experimental · 2022Review
- MicroRNA expression profile in the spinal cord injured rat neurogenic bladder by next-generation sequencing.Translational andrology and urology · 2020Article
- The Inhibitory Mechanism on Acetylcholine-Induced Contraction of Bladder Smooth Muscle in the Streptozotocin-Induced Diabetic Rat.Biomolecules & therapeutics · 2019Article
- Regulation of Spontaneous Contractions in Intact Rat Bladder Strips and the Effects of Hydrogen Peroxide.BioMed research international · 2018Article
- Article
- Effects of daily tadalafil on lower urinary tract symptoms in young men with multiple sclerosis and erectile dysfunction: a pilot study.Journal of endocrinological investigation · 2017Article
- Effect of phosphodiesterase inhibitors in the bladder.Asian journal of urology · 2015Review
- Nitric oxide modulates bladder afferent nerve activity in the in vitro urinary bladder-pelvic nerve preparation from rats with cyclophosphamide induced cystitis.Brain research · 2013Article
- Evidence for the role of mast cells in colon-bladder cross organ sensitization.Autonomic neuroscience : basic & clinical · 2013Article
- Common theme for drugs effective in overactive bladder treatment: inhibition of afferent signaling from the bladder.International journal of urology : official journal of the Japanese Urological Association · 2013Review
- Nitro-oleic acid targets transient receptor potential (TRP) channels in capsaicin sensitive afferent nerves of rat urinary bladder.Experimental neurology · 2011Article
- Developmental and spinal cord injury-induced changes in nitric oxide-mediated inhibition in rat urinary bladder.Neurourology and urodynamics · 2011Article
- Differential effect of L-cysteine in isolated whole-bladder preparations from neonatal and adult rats.The Journal of pharmacology and experimental therapeutics · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Nitric oxide (NO), a neurotransmitter in the lower urinary tract, stimulates soluble guanylyl cyclase (sGC) and in turn cGMP-dependent protein kinase G (PKG) to modulate a number of downstream targets. NO donors reduce bladder hyperactivity in some pathological models but do not affect normal bladder activity in the adult rat. In this study, the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP; 100 microM) decreased the amplitude and frequency of spontaneous and carbachol-enhanced contractions in neonatal rat bladder strips, which are intrinsically hyperactive. This effect was blocked by inhibition of sGC and mimicked by application of a membrane-permeable cGMP analog (8-bromo-cGMP, 100 microM). Inhibition of PKG prevented or reversed the inhibitory effects of 8-bromo-cGMP. A portion of the SNAP-mediated inhibition was also dependent upon PKG; however, a short-lasting, sGC-dependent inhibitory effect of SNAP was still present after PKG inhibition. Inhibition of NO synthase with L-NAME (100 microM) did not change the amplitude or frequency of contractions. However, inhibition of endogenous phosphodiesterase (PDE)-5 with zaprinast (25 microM) reduced the amplitude and frequency of phasic contractions and increased the magnitude of inhibition produced by maximal concentrations of SNAP, suggesting that endogenous PDEs are constitutively active and regulate cGMP production. These results suggest that the NO-cGMP-PKG pathway may be involved in inhibitory control of the neonatal rat bladder.
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