ArticleBiochemistry2009
Evidence for two retinoid cycles in the cone-dominated chicken eye.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Retinal pigment epithelium 65 kDa protein (RPE65): An update.Progress in retinal and eye research · 2022Review
- Expression of Pro-Angiogenic Markers Is Enhanced by Blue Light in Human RPE Cells.Antioxidants (Basel, Switzerland) · 2020Article
- The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration.International journal of molecular sciences · 2020Article
- Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis.Cell cycle (Georgetown, Tex.) · 2019Article
- Visual cycle proteins: Structure, function, and roles in human retinal disease.The Journal of biological chemistry · 2018Article
- Retinoid isomerase inhibitors impair but do not block mammalian cone photoreceptor function.The Journal of general physiology · 2018Article
- Retinoids and Retinal Diseases.Annual review of vision science · 2016Review
- CRALBP supports the mammalian retinal visual cycle and cone vision.The Journal of clinical investigation · 2015Article
- Interphotoreceptor retinoid-binding protein (IRBP) promotes retinol uptake and release by rat Müller cells (rMC-1) in vitro: implications for the cone visual cycle.Investigative ophthalmology & visual science · 2014Article
- Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina.Nature chemical biology · 2013Article
- New insights into retinoid metabolism and cycling within the retina.Progress in retinal and eye research · 2013Review
- Cultured Müller cells from mammals can synthesize and accumulate retinyl esters.Experimental eye research · 2012Article
- Cone outer segment extracellular matrix as binding domain for interphotoreceptor retinoid-binding protein.The Journal of comparative neurology · 2012Article
- RPE65 is present in human green/red cones and promotes photopigment regeneration in an in vitro cone cell model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- An alternative isomerohydrolase in the retinal Müller cells of a cone-dominant species.The FEBS journal · 2011Article
- Regeneration of photopigment is enhanced in mouse cone photoreceptors expressing RPE65 protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Gene therapy rescues cone structure and function in the 3-month-old rd12 mouse: a model for midcourse RPE65 leber congenital amaurosis.Investigative ophthalmology & visual science · 2011Article
- Membrane-binding and enzymatic properties of RPE65.Progress in retinal and eye research · 2010Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In the classic retinoid cycle, 11-cis retinol is synthesized in the retinal pigment epithelium (RPE) by two enzymes: Isomerase I (RPE65) and lecithin:retinol acyltransferase (LRAT). The purpose of this study is to provide experimental evidence for two active isomerases in the cone-dominated chicken eye: an LRAT-dependent Isomerase I in the RPE and an ARAT (acyl CoA:retinol acyltransferase)-dependent isomerase (Isomerase II) in the retina. First, we show that whole chicken retina in vitro, removed from the RPE/choroid and sclera, produces 11-cis retinoids upon light exposure, indicating the existence of RPE-independent isomerase (Isomerase II) activity in the retina. Reverse transcriptase polymerase chain reaction studies show high levels of RPE65 expression in the RPE, low levels in the retina, and none in primary Muller cell cultures, indicating the presence of Isomerase I in the RPE and a minimal amount in the retina. Activities of the RPE and retina isomerases were then measured by enzyme assays with specific enzyme inhibitors. 2,2'-Bipyridine, a known Isomerase I inhibitor, and N-ethylmaleimide (NEM), a known LRAT inhibitor, significantly reduced Isomerase I activity but not Isomerase II activity. Progesterone, a known ARAT inhibitor, completely blocked Isomerase II activity but not Isomerase I activity. Thus, this study reports novel results for distinguishing the biochemical properties of Isomerase I from those of Isomerase II, as well a difference in their locations in the chicken eye. On the basis of these differences, the cone-dominated chicken eye must contain two retinoid cycles: a classic visual cycle for retinoid exchange between the RPE and the retina supported by Isomerase I in the RPE and an additional visual cycle for retinoid processing in the retina supported by Isomerase II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.