ArticlePloS one2009
Conserved threonine residues within the A-loop of the receptor NIK differentially regulate the kinase function required for antiviral signaling.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- NIK receptor kinases propagate phytocytokine-triggered immunity by driving early phosphorylation events.Nature plants · 2026Article
- Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance.Plant molecular biology · 2026Article
- Defence-mediated phloem restriction of a plant virus facilitates insect transmission.Nature communications · 2025Article
- Deep learning in the discovery of antiviral peptides and peptidomimetics: databases and prediction tools.Molecular diversity · 2025Review
- The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.Nature communications · 2025Article
- LRR-RLK subfamily II of coreceptors: emerging, non-canonical and canonical roles in plant antiviral immunity and development.Frontiers in plant science · 2025Review
- Transcriptomic profiling reveals distinct responses to beet curly top virus (BCTV) infection in resistant and susceptible sugar beet genotypes.BMC genomics · 2024Article
- Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana.Nature communications · 2024Article
- The osmotic stress-activated receptor-like kinase DPY1 mediates SnRK2 kinase activation and drought tolerance in Setaria.The Plant cell · 2023Article
- Review
- The COG1-OsSERL2 complex senses cold to trigger signaling network for chilling tolerance in japonica rice.Nature communications · 2023Article
- Review
- RLPredictiOme, a Machine Learning-Derived Method for High-Throughput Prediction of Plant Receptor-like Proteins, Reveals Novel Classes of Transmembrane Receptors.International journal of molecular sciences · 2022Article
- Proteome Mapping of South African Cassava Mosaic Virus-Infected Susceptible and Tolerant Landraces of Cassava.Proteomes · 2021Article
- Activation loop phosphorylation of a non-RD receptor kinase initiates plant innate immune signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV.Plant physiology · 2021Article
- Plant responses to geminivirus infection: guardians of the plant immunity.Virology journal · 2021Review
- Geminivirus-Host Interactions: Action and Reaction in Receptor-Mediated Antiviral Immunity.Viruses · 2021Review
- Geminiviral Triggers and Suppressors of Plant Antiviral Immunity.Microorganisms · 2021Review
- A Begomovirus Nuclear Shuttle Protein-Interacting Immune Hub: Hijacking Host Transport Activities and Suppressing Incompatible Functions.Frontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NSP-interacting kinase (NIK1) is a receptor-like kinase identified as a virulence target of the begomovirus nuclear shuttle protein (NSP). We found that NIK1 undergoes a stepwise pattern of phosphorylation within its activation-loop domain (A-loop) with distinct roles for different threonine residues. Mutations at Thr-474 or Thr-468 impaired autophosphorylation and were defective for kinase activation. In contrast, a mutation at Thr-469 did not impact autophosphorylation and increased substrate phosphorylation, suggesting an inhibitory role for Thr-469 in kinase function. To dissect the functional significance of these results, we used NSP-expressing virus infection as a mechanism to interfere with wild type and mutant NIK1 action in plants. The NIK1 knockout mutant shows enhanced susceptibility to virus infections, a phenotype that could be complemented with ectopic expression of a 35S-NIK1 or 35S-T469A NIK1 transgenes. However, ectopic expression of an inactive kinase or the 35S-T474A NIK1 mutant did not reverse the enhanced susceptibility phenotype of knockout lines, demonstrating that Thr-474 autophosphorylation was needed to transduce a defense response to geminiviruses. Furthermore, mutations at Thr-474 and Thr-469 residues antagonistically affected NIK-mediated nuclear relocation of the downstream effector rpL10. These results establish that NIK1 functions as an authentic defense receptor as it requires activation to elicit a defense response. Our data also suggest a model whereby phosphorylation-dependent activation of a plant receptor-like kinase enables the A-loop to control differentially auto- and substrate phosphorylation.
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