ArticleActa histochemica et cytochemica2009
The influence of endocrine disrupting chemicals on the proliferation of ERalpha knockdown-human breast cancer cell line MCF-7; new attempts by RNAi technology.
Article in Acta histochemica et cytochemica, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Developmental neurotoxicity of bisphenol F and bisphenol S in animal model systems: A literature review.Neurotoxicology · 2025Review
- Roles of estrogens, estrogen-like compounds, and endocrine disruptors in adipocytes.Frontiers in endocrinology · 2022Review
- Effect of bisphenols on telomerase expression and activity in breast cancer cell lines.Molecular biology reports · 2020Article
- Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats.Clinical and experimental medicine · 2017Article
- Bisphenol A Induces Sox2 in ERHormones & cancer · 2017Article
- Methylparaben stimulates tumor initiating cells in ER+ breast cancer models.Journal of applied toxicology : JAT · 2017Article
- Article
- Bisphenol A alters β-hCG and MIF release by human placenta: an in vitro study to understand the role of endometrial cells.Mediators of inflammation · 2014Article
- Molecular mechanism(s) of endocrine-disrupting chemicals and their potent oestrogenicity in diverse cells and tissues that express oestrogen receptors.Journal of cellular and molecular medicine · 2013Review
- The effects of maternal exposure to bisphenol A on allergic lung inflammation into adulthood.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Estrogen-induced memory enhancements are blocked by acute bisphenol A in adult female rats: role of dendritic spines.Endocrinology · 2012Article
- Prenatal and Lactational Exposure to Bisphenol A in Mice Alters Expression of Genes Involved in Cortical Barrel Development without Morphological Changes.Acta histochemica et cytochemica · 2011Article
- The expression of Wnt4 is regulated by estrogen via an estrogen receptor alpha-dependent pathway in rat pituitary growth hormone-producing cells.Acta histochemica et cytochemica · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bisphenol A (BPA) is a monomer use in manufacturing a wide range of chemical products which include epoxy resins and polycarbonate. It has been reported that BPA increases the cell proliferation activity of human breast cancer MCF-7 cells as well as 17-beta estradiol (E2) and diethylstilbestrol (DES). However, BPA induces target genes through ER-dependent and ER-independent manners which are different from the actions induced by E2. Therefore, BPA may be unique in estrogen-dependent cell proliferation compared to other endocrine disrupting chemicals (EDCs). In the present study, to test whether ERalpha is essential to the BPA-induced proliferation on MCF-7 cells, we suppressed the ERalpha expression of MCF-7 cells by RNA interference (RNAi). Proliferation effects in the presence of E2, DES and BPA were not observed in ERalpha-knockdown MCF-7 cells in comparison with control MCF-7. In addition, a marker of proliferative potential, MIB-1 labeling index (LI), showed no change in BPA-treated groups compared with vehicle-treated groups on ERalpha-knockdown MCF-7 cells. In conclusion, we demonstrated that ERalpha has a role in BPA-induced cell proliferation as well as E2 and DES. Moreover, this study indicated that the direct knockdown of ERalpha using RNAi serves as an additional tool to evaluate, in parallel with MCF-7 cell proliferation assay, for potential EDCs.
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