ArticlePloS one2009
Effect of Ca(v)beta subunits on structural organization of Ca(v)1.2 calcium channels.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Vascular smooth muscle ion channels in essential hypertension.Frontiers in physiology · 2022Review
- Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation.PloS one · 2018Article
- Electrophysiological evidences of interaction between calcium channels and PA of anthrax.Channels (Austin, Tex.) · 2016Article
- Down-regulation of CaV1.2 channels during hypertension: how fewer CaV1.2 channels allow more Ca(2+) into hypertensive arterial smooth muscle.The Journal of physiology · 2013Article
- Cav1.2, cell proliferation, and new target in atherosclerosis.ISRN biochemistry · 2013Review
- Molecular Determinants of Cav1.2 Calcium Channel Inactivation.ISRN molecular biology · 2012Review
- Microdomain organization and frequency-dependence of CREB-dependent transcriptional signaling in heart cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Oligomerization of Cavbeta subunits is an essential correlate of Ca2+ channel activity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Selective fluorophore-assisted light inactivation of voltage-gated calcium channels.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundVoltage-gated Ca(v)1.2 calcium channels play a crucial role in Ca(2+) signaling. The pore-forming alpha(1C) subunit is regulated by accessory Ca(v)beta subunits, cytoplasmic proteins of various size encoded by four different genes (Ca(v)beta(1)-beta(4)) and expressed in a tissue-specific manner. METHODS AND
resultsHere we investigated the effect of three major Ca(v)beta types, beta(1b), beta(2d) and beta(3), on the structure of Ca(v)1.2 in the plasma membrane of live cells. Total internal reflection fluorescence microscopy showed that the tendency of Ca(v)1.2 to form clusters depends on the type of the Ca(v)beta subunit present. The highest density of Ca(v)1.2 clusters in the plasma membrane and the smallest cluster size were observed with neuronal/cardiac beta(1b) present. Ca(v)1.2 channels containing beta(3), the predominant Ca(v)beta subunit of vascular smooth muscle cells, were organized in a significantly smaller number of larger clusters. The inter- and intramolecular distances between alpha(1C) and Ca(v)beta in the plasma membrane of live cells were measured by three-color FRET microscopy. The results confirm that the proximity of Ca(v)1.2 channels in the plasma membrane depends on the Ca(v)beta type. The presence of different Ca(v)beta subunits does not result in significant differences in the intramolecular distance between the termini of alpha(1C), but significantly affects the distance between the termini of neighbor alpha(1C) subunits, which varies from 67 A with beta(1b) to 79 A with beta(3).
conclusionsThus, our results show that the structural organization of Ca(v)1.2 channels in the plasma membrane depends on the type of Ca(v)beta subunits present.
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