ArticleThe Journal of biological chemistry2009
Combinatorial transcription factor regulation of the cyclic AMP-response element on the Pgc-1alpha promoter in white 3T3-L1 and brown HIB-1B preadipocytes.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- SOX4 promotes beige adipocyte-mediated adaptive thermogenesis by facilitating PRDM16-PPARγ complex.Theranostics · 2022Article
- Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats.International journal of medical sciences · 2022Article
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- Identification of Estrogen-Related Receptor α Agonists in the Tox21 Compound Library.Endocrinology · 2018Article
- Endoplasmic Reticulum Stress-Induced CHOP Inhibits PGC-1α and Causes Mitochondrial Dysfunction in Diabetic Embryopathy.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Transcriptional and epigenetic control of brown and beige adipose cell fate and function.Nature reviews. Molecular cell biology · 2016Review
- ATF4 deficiency protects hepatocytes from oxidative stress via inhibiting CYP2E1 expression.Journal of cellular and molecular medicine · 2014Article
- Association of single nucleotide polymorphism Rs2236518 in PRDM16 gene with BMI in Chinese males.Acta pharmacologica Sinica · 2013Article
- Synergism between cAMP and PPARγ Signalling in the Initiation of UCP1 Gene Expression in HIB1B Brown Adipocytes.PPAR research · 2013Article
- Cold-induced changes in gene expression in brown adipose tissue, white adipose tissue and liver.PloS one · 2013Article
- Tyk2 and Stat3 regulate brown adipose tissue differentiation and obesity.Cell metabolism · 2012Article
- Lipolytic products activate peroxisome proliferator-activated receptor (PPAR) α and δ in brown adipocytes to match fatty acid oxidation with supply.The Journal of biological chemistry · 2012Article
- A comparative approach to understanding tissue-specific expression of uncoupling protein 1 expression in adipose tissue.Frontiers in genetics · 2012Article
- Article
- NR4A orphan nuclear receptors as mediators of CREB-dependent neuroprotection.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Role of Ucp1 enhancer methylation and chromatin remodelling in the control of Ucp1 expression in murine adipose tissue.Diabetologia · 2010Article
- Transcriptional control of brown fat development.Cell metabolism · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cold stress in rodents increases the expression of UCP1 and PGC-1alpha in brown and white adipose tissue. We have previously reported that C/EBPbeta specifically binds to the CRE on the proximal Pgc-1alpha promoter and increases forskolin-sensitive Pgc-1alpha and Ucp1 expression in white 3T3-L1 preadipocytes. Here we show that in mice exposed to a cold environment for 24 h, Pgc-1alpha, Ucp1, and C/ebpbeta but not C/ebpalpha or C/ebpdelta expression were increased in BAT. Conversely, expression of the C/EBP dominant negative Chop10 was increased in WAT but not BAT during cold exposure. Reacclimatization of cold-exposed mice to a warm environment for 24 h completely reversed these changes in gene expression. In HIB-1B, brown preadipocytes, forskolin increased expression of Pgc-1alpha, Ucp1, and C/ebpbeta early in differentiation and inhibited Chop10 expression. Employing chromatin immunoprecipitation, we demonstrate that C/EBPbeta, CREB, ATF-2, and CHOP10 are bound to the Pgc-1alpha proximal CRE, but CHOP10 does not bind in HIB-1B cell lysates. Forskolin stimulation and C/EBPbeta overexpression in 3T3-L1 cells increased C/EBPbeta and CREB but displaced ATF-2 and CHOP10 binding to the Pgc-1alpha proximal CRE. Overexpression of ATF-2 and CHOP10 in 3T3-L1 cells decreased Pgc-1alpha transcription. Knockdown of Chop10 in 3T3-L1 cells using siRNA increased Pgc-1alpha transcription, whereas siRNA against C/ebpbeta in HIB-1B cells decreased Pgc-1alpha and Ucp1 expression. We conclude that the increased cAMP stimulation of Pgc-1alpha expression is regulated by the combinatorial effect of transcription factors acting at the CRE on the proximal Pgc-1alpha promoter.
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