ArticleMicrobial pathogenesis2009
Intercellular adhesion and biocide resistance in nontypeable Haemophilus influenzae biofilms.
Article in Microbial pathogenesis, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 77 citations in OpenAlex.
- Natural Anti-Biofilm Agents: A Comprehensive Review and Future Perspectives.Current microbiology · 2025Review
- Secreted nucleases reclaim extracellular DNA during biofilm development.NPJ biofilms and microbiomes · 2024Article
- Oxidative stress responses in biofilms.Biofilm · 2024Review
- Multiple biological characteristics and functions of intestinal biofilm extracellular polymers: friend or foe?Frontiers in microbiology · 2024Review
- Case Report: Whole-Genome Sequencing of Serially CollectedFrontiers in cellular and infection microbiology · 2022Article
- The Role of Non-TypeableFrontiers in cellular and infection microbiology · 2021Review
- The DmsABC Sulfoxide Reductase Supports Virulence in Non-typeableFrontiers in microbiology · 2021Article
- Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles.Microorganisms · 2020Review
- Deoxyribonucleases and Their Applications in Biomedicine.Biomolecules · 2020Review
- Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens.Frontiers in microbiology · 2020Review
- Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and PersistenceInfection and immunity · 2018Article
- Antibiofilm agents: A new perspective for antimicrobial strategy.Journal of microbiology (Seoul, Korea) · 2017Review
- Haemophilus parainfluenzae Strain ATCC 33392 Forms BiofilmsInfection and immunity · 2017Article
- Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease.Pathogens and disease · 2017Review
- Approaches to Dispersing Medical Biofilms.Microorganisms · 2017Review
- Resistance of non-typeable Haemophilus influenzae biofilms is independent of biofilm size.Pathogens and disease · 2017Article
- Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities.Frontiers in microbiology · 2017Review
- What's on the Outside Matters: The Role of the Extracellular Polymeric Substance of Gram-negative Biofilms in Evading Host Immunity and as a Target for Therapeutic Intervention.The Journal of biological chemistry · 2016Review
- A Novel, Molybdenum-Containing Methionine Sulfoxide Reductase Supports Survival ofFrontiers in microbiology · 2016Article
- Comparative analyses of proteins from Haemophilus influenzae biofilm and planktonic populations using metabolic labeling and mass spectrometry.BMC microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Respiratory infections caused by nontypeable Haemophilus influenzae (NTHi) are a major medical problem. Evidence suggests that the ability to form biofilms on mucosal surfaces may play a role in NTHi pathogenesis. However, the factors that contribute to NTHi biofilm cohesion remain largely unknown. In this study we investigated the biofilm growth and detachment phenotypes of eight NTHi clinical strains in vitro. We found that the majority of strains produced biofilms within 6h when cultured statically in tubes. Biofilm formation was inhibited when culture medium was supplemented with proteinase K or DNase I. Both enzymes also caused significant detachment of pre-formed NTHi biofilms. These findings indicate that both proteinaceous adhesins and extracellular DNA contribute to NTHi biofilm cohesion. Treatment of NTHi biofilms cultured in centrifugal filter devices with DNase I, but not with proteinase K, caused a significant decrease in fluid convection through the biofilms. These results suggest that extracellular DNA is the major volumetric component of the NTHi biofilm matrix. Mechanical or enzymatic disruption of NTHi biofilms cultured in microtiter plates significantly increased their sensitivity to killing by SDS, cetylpyridinium chloride, chlorhexidine gluconate, povidone iodine and sodium hypochlorite. These findings indicate that biocide resistance in NTHi biofilms is mediated to a large part by the cohesive and protective properties of the biofilm matrix. Understanding the mechanisms of biofilm cohesion and biocide resistance in NTHi biofilms may lead to new methods for treating NTHi-associated infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.