ArticleMicrobial cell factories2009
Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris.
Article in Microbial cell factories, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 226 citations in OpenAlex.
- A New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration and Partial Purification of Recombinant Prepromelittin Produced by Komagataella phaffii.Biotechnology and applied biochemistry · 2026Article
- Simultaneous GCN4 co-expression and promoter optimization enhance glucose oxidase production inIranian journal of microbiology · 2026Article
- Pichia pastoris Genomic Resources: Accessibility, Recommendations, and Pitfalls.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Decoding the Genome of the Komagataella phaffii Type Strain CBS 2612.Yeast (Chichester, England) · 2025Article
- Primary high-throughput screening of engineered phytases by online monitoring of the oxygen transfer rate of Komagataella phaffii.Microbial cell factories · 2025Article
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.FEMS yeast research · 2025Review
- Glycerol bioconversion to biofuel and value-added products by yeasts.FEMS yeast research · 2025Review
- Identification and functional analysis of growth rate associated long non-coding RNAs inComputational and structural biotechnology journal · 2025Article
- Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.Microbial cell factories · 2024Article
- Avoiding overflow metabolite formation in Komagataella phaffii fermentations to enhance recombinant protein production.Journal of biological engineering · 2024Article
- Improvement of the recombinant phytase expression by intermittent feeding of glucose during the induction phase of methylotrophic yeast Pichia pastoris.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Exploring the secretome ofFrontiers in bioengineering and biotechnology · 2024Article
- Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission.Parasitology research · 2023Article
- Affinity-based and in a single step purification of recombinant horseradish peroxidase A2A isoenzyme produced by Pichia pastoris.Bioprocess and biosystems engineering · 2023Article
- Effect ofMolecules (Basel, Switzerland) · 2023Article
- Loss of a Functional Mitochondrial Pyruvate Carrier inMicroorganisms · 2023Article
- Enabling growth-decoupledFrontiers in bioengineering and biotechnology · 2023Article
- Insights into the glycerol transport of Yarrowia lipolytica.Yeast (Chichester, England) · 2022Article
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- Enhancement of HSA-pFSHβ production by disruptingFrontiers in microbiology · 2022Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPichia pastoris is widely used as a production platform for heterologous proteins and model organism for organelle proliferation. Without a published genome sequence available, strain and process development relied mainly on analogies to other, well studied yeasts like Saccharomyces cerevisiae.
resultsTo investigate specific features of growth and protein secretion, we have sequenced the 9.4 Mb genome of the type strain DSMZ 70382 and analyzed the secretome and the sugar transporters. The computationally predicted secretome consists of 88 ORFs. When grown on glucose, only 20 proteins were actually secreted at detectable levels. These data highlight one major feature of P. pastoris, namely the low contamination of heterologous proteins with host cell protein, when applying glucose based expression systems. Putative sugar transporters were identified and compared to those of related yeast species. The genome comprises 2 homologs to S. cerevisiae low affinity transporters and 2 to high affinity transporters of other Crabtree negative yeasts. Contrary to other yeasts, P. pastoris possesses 4 H+/glycerol transporters.
conclusionThis work highlights significant advantages of using the P. pastoris system with glucose based expression and fermentation strategies. As only few proteins and no proteases are actually secreted on glucose, it becomes evident that cell lysis is the relevant cause of proteolytic degradation of secreted proteins. The endowment with hexose transporters, dominantly of the high affinity type, limits glucose uptake rates and thus overflow metabolism as observed in S. cerevisiae. The presence of 4 genes for glycerol transporters explains the high specific growth rates on this substrate and underlines the suitability of a glycerol/glucose based fermentation strategy. Furthermore, we present an open access web based genome browser http://www.pichiagenome.org.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.