ArticleStem cells (Dayton, Ohio)2009
Reprogramming retinal pigment epithelium to differentiate toward retinal neurons with Sox2.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Spatiotemporal characterization of a distinct nectin-3Scientific reports · 2026Article
- Article
- Pigment Epithelia of the Eye: Cell-Type Conversion in Regeneration and Disease.Life (Basel, Switzerland) · 2022Review
- Retina regeneration: lessons from vertebrates.Oxford open neuroscience · 2022Review
- Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing.International journal of molecular sciences · 2021Article
- Deficiency of C-X-C chemokine receptor type 5 (CXCR5) gene causes dysfunction of retinal pigment epithelium cells.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Lin28 reprograms inner ear glia to a neuronal fate.Stem cells (Dayton, Ohio) · 2020Article
- Clinical and molecular markers in retinal detachment-From hyperreflective points to stem cells and inflammation.PloS one · 2019Article
- Molecular Mechanisms Mediating Diabetic Retinal Neurodegeneration: Potential Research Avenues and Therapeutic Targets.Journal of molecular neuroscience : MN · 2018Review
- The microRNA expression profile of mouse Müller glia in vivo and in vitro.Scientific reports · 2016Article
- Reprogramming human retinal pigmented epithelial cells to neurons using recombinant proteins.Stem cells translational medicine · 2014Article
- The newt reprograms mature RPE cells into a unique multipotent state for retinal regeneration.Scientific reports · 2014Article
- Retinal pigment epithelium development, plasticity, and tissue homeostasis.Experimental eye research · 2014Review
- Amniotic fluid promotes the appearance of neural retinal progenitors and neurons in human RPE cell cultures.Molecular vision · 2013Article
- Choice of Cell Source in Cell-Based Therapies for Retinal Damage due to Age-Related Macular Degeneration: A Review.Journal of ophthalmology · 2013Article
- Generation of induced pluripotent stem cells from human Tenon's capsule fibroblasts.Molecular vision · 2012Article
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Cytoskeleton proteins previously considered exclusive to ganglion cells are transiently expressed by all retinal neuronal precursors.BMC developmental biology · 2011Article
- Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction.PloS one · 2011Article
- Generating retinal neurons by reprogramming retinal pigment epithelial cells.Expert opinion on biological therapy · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Guiding non-neural, retinal pigment epithelium (RPE) to produce retinal neurons may offer a source of developing neurons for cell-replacement. Sox2 plays important roles in maintaining neural progenitor/stem cell properties and in converting fibroblasts into pluripotent stem cells. This study tests the possibility of using Sox2 to reprogram RPE to differentiate toward retinal neurons in vivo and in vitro. Expression of Sox2 in the chick retina was detected in progenitor cells, in cells at a discrete location in the layers of amacrine and ganglion cells, and in Muller glia. Overexpression of Sox2 in the developing eye resulted in hypopigmentation of the RPE. In the affected regions, expression of retinal ganglion cell markers became apparent in the RPE layer. In RPE cell culture, Sox2 promoted the expression of retinal ganglion and amacrine markers, and suppressed the expression of genes associated with RPE properties. Mechanistic investigation using the developing retina revealed a coexpression of Sox2 and basic fibroblast growth factor (bFGF), a growth factor commonly used in stem cell culture and capable of inducing RPE-to-retina transdifferentiation (or reprogramming) during early development. Similar patterns of changes in Sox2 expression and in bFGF expression were observed in atrophic retina and in injured retina. In RPE cell culture, Sox2 and bFGF mutually enhanced one another's expression. Upregulation of bFGF expression by Sox2 also occurred in the retina. These results suggest that Sox2 can initiate a reprogramming of RPE cells to differentiate toward retinal neurons and may engage bFGF during the process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.