ArticlePlanta2009
Multiplicity of aspartic proteinases from Cynara cardunculus L.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Production of Protein Hydrolysates Teff (Current issues in molecular biology · 2024Article
- Bioactive Compounds from Artichoke and Application Potential.Food technology and biotechnology · 2023Review
- Clotting and Proteolytic Activity of Freeze-Dried Crude Extracts Obtained from Wild ThistlesFoods (Basel, Switzerland) · 2023Article
- Safety evaluation of the food enzyme phytepsin fromEFSA journal. European Food Safety Authority · 2023Article
- Safety evaluation of the food enzyme phytepsin fromEFSA journal. European Food Safety Authority · 2022Article
- Safety evaluation of the food enzyme phytepsin fromEFSA journal. European Food Safety Authority · 2022Article
- Safety evaluation of the food enzyme phytepsin fromEFSA journal. European Food Safety Authority · 2022Article
- The Employment of GeneraNutrients · 2022Review
- Plant Milk-Clotting Enzymes for Cheesemaking.Foods (Basel, Switzerland) · 2022Review
- Bioactive Compounds from Cardoon as Health Promoters in Metabolic Disorders.Foods (Basel, Switzerland) · 2022Review
- Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes.Scientific reports · 2021Article
- Exploring the Milk-Clotting and Proteolytic Activities in Different Tissues of Vallesia glabra: a New Source of Plant Proteolytic Enzymes.Applied biochemistry and biotechnology · 2021Article
- Genetic diversity and population structure of Cynara cardunculus L. in southern Portugal.PloS one · 2021Article
- Plant Aspartic Proteases for Industrial Applications: Thistle Get Better.Plants (Basel, Switzerland) · 2020Review
- Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L.Plant cell reports · 2020Article
- Technological characterization and sensory evaluation of a traditional Algerian fresh cheese clotted withJournal of food science and technology · 2019Article
- Identification of proteins from wild cardoon flowers (Journal of chemical biology · 2017Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspartic proteinases (AP) play major roles in physiologic and pathologic scenarios in a wide range of organisms from vertebrates to plants or viruses. The present work deals with the purification and characterisation of four new APs from the cardoon Cynara cardunculus L., bringing the number of APs that have been isolated, purified and biochemically characterised from this organism to nine. This is, to our knowledge, one of the highest number of APs purified from a single organism, consistent with a specific and important biological function of these protein within C. cardunculus. These enzymes, cardosins E, F, G and H, are dimeric, glycosylated, pepstatin-sensitive APs, active at acidic pH, with a maximum activity around pH 4.3. Their primary structures were partially determined by N- and C-terminal sequence analysis, peptide mass fingerprint analysis on a MALDI-TOF/TOF instrument and by LC-MS/MS analysis on a Q-TRAP instrument. All four enzymes are present on C. cardunculus L. pistils, along with cyprosins and cardosins A and B. Their micro-heterogeneity was detected by 2D-electrophoresis and mass spectrometry. The enzymes resemble cardosin A more than they resemble cardosin B or cyprosin, with cardosin E and cardosin G being more active than cardosin A, towards the synthetic peptide KPAEFF(NO(2))AL. The specificity of these enzymes was investigated and it is shown that cardosin E, although closely related to cardosin A, exhibits different specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.