ArticleJournal of visualized experiments : JoVE2009
Harvesting and preparing Drosophila embryos for electrophysiological recording and other procedures.
Article in Journal of visualized experiments : JoVE, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- The larvalScience advances · 2026Article
- The larvalbioRxiv : the preprint server for biology · 2026Article
- Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad.Journal of visualized experiments : JoVE · 2020Article
- Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes.Journal of visualized experiments : JoVE · 2020Article
- Assessment of mitophagy in mt-KeimaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Starvation resistance is associated with developmentally specified changes in sleep, feeding and metabolic rate.The Journal of experimental biology · 2019Article
- Article
- The Drosophila neurogenin Tap functionally interacts with the Wnt-PCP pathway to regulate neuronal extension and guidance.Development (Cambridge, England) · 2016Article
- The Extracellular-Regulated Kinase Effector Lk6 is Required for Glutamate Receptor Localization at the Drosophila Neuromuscular Junction.Journal of experimental neuroscience · 2016Article
- Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1.The Journal of cell biology · 2014Article
- Labeling of single cells in the central nervous system of Drosophila melanogaster.Journal of visualized experiments : JoVE · 2013Article
- Structure-function analysis of endogenous lectin mind-the-gap in synaptogenesis.Developmental neurobiology · 2012Article
- Morphological analysis of Drosophila larval peripheral sensory neuron dendrites and axons using genetic mosaics.Journal of visualized experiments : JoVE · 2011Article
- Membrane penetration by synaptotagmin is required for coupling calcium binding to vesicle fusion in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Anterograde Jelly belly ligand to Alk receptor signaling at developing synapses is regulated by Mind the gap.Development (Cambridge, England) · 2010Article
- 100 years of Drosophila research and its impact on vertebrate neuroscience: a history lesson for the future.Nature reviews. Neuroscience · 2010Review
- Preparation of developing and adult Drosophila brains and retinae for live imaging.Journal of visualized experiments : JoVE · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Drosophila is a premier genetic model for the study of both embryonic development and functional neuroscience. Traditionally, these fields are quite isolated from each other, with largely independent histories and scientific communities. However, the interface between these usually disparate fields is the developmental programs underlying acquisition of functional electrical signaling properties and differentiation of functional chemical synapses during the final phases of neural circuit formation. This interface is a critically important area for investigation. In Drosophila, these phases of functional development occur during a period of <8 hours (at 25 degrees C) during the last third of embryogenesis. This late developmental period was long considered intractable to investigation owing to the deposition of a tough, impermeable epidermal cuticle. A breakthrough advance was the application of water-polymerizing surgical glue that can be locally applied to the cuticle to enable controlled dissection of late-stage embryos. With a dorsal longitudinal incision, the embryo can be laid flat, exposing the ventral nerve cord and body wall musculature to experimental investigation. This system has been heavily used to isolate and characterize genetic mutants that impair embryonic synapse formation, and thus reveal the molecular mechanisms governing the specification and differentiation of synapse connections and functional synaptic signaling properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.