ArticleThe Journal of biological chemistry2009
House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- CPP Applications in Immune Modulation and Disease Therapy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nociceptor neurons promote IgE class switch in B cells.JCI insight · 2021Article
- Review
- The effects of cigarette smoking extracts on cell cycle and tumor spread: novel evidence.Future science OA · 2019Review
- Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model.Experimental & molecular medicine · 2018Article
- Phospholipase D1 Signaling: Essential Roles in Neural Stem Cell Differentiation.Journal of molecular neuroscience : MN · 2018Review
- Leptin increases TNF-α expression and production through phospholipase D1 in Raw 264.7 cells.PloS one · 2014Article
- Influence of the Adjuvants and Genetic Background on the Asthma Model Using Recombinant Der f 2 in Mice.Immune network · 2013Article
- House dust mite allergen Der f 2 induces interleukin-13 expression by activating the PI3K/Akt pathway.Immunologic research · 2013Article
- Article
- Innate immune responses in house dust mite allergy.ISRN allergy · 2013Article
- House dust mite allergy in Korea: the most important inhalant allergen in current and future.Allergy, asthma & immunology research · 2012Article
- Phospholipase D1 is an important regulator of bFGF-induced neurotrophin-3 expression and neurite outgrowth in H19-7 cells.Molecular neurobiology · 2012Article
- A Single Nucleotide Polymorphism in the Phospholipase D1 Gene is Associated with Risk of Non-Small Cell Lung Cancer.International journal of biomedical science : IJBS · 2012Article
- Allergen particle binding by human primary bronchial epithelial cells is modulated by surfactant protein D.Respiratory research · 2010Article
- Household arthropod allergens in Korea.The Korean journal of parasitology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Der f 2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f 2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f 2-induced IL-13 expression and production. Treatment of Der f 2 activated the phospholipase Cgamma (PLCgamma)/protein kinase Calpha (PKCalpha)/p38 MAPK pathway. Der f 2-induced PLD activation was attenuated by PLCgamma inhibitors (U73122 and PAO), PKCalpha inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLCgamma, PKCalpha, and p38 MAPK act as upstream activators of PLD in Der f 2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f 2 were also blocked by inhibition of PLCgamma, PKCalpha, or p38 MAPK, indicating that IL-13 expression and production are related to a PLCgamma/PKCalpha/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f 2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f 2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f 2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f 2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.