ArticleThe Journal of biological chemistry2009
Sorting nexin 33 induces mammalian cell micronucleated phenotype and actin polymerization by interacting with Wiskott-Aldrich syndrome protein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Identification of GOLPH3 Partners inCells · 2021Article
- EnteropathogenicInternational journal of molecular sciences · 2020Article
- BAR domain proteins-a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton.Biophysical reviews · 2018Review
- Attaching-and-Effacing Pathogens Exploit Junction Regulatory Activities of N-WASP and SNX9 to Disrupt the Intestinal Barrier.Cellular and molecular gastroenterology and hepatology · 2018Article
- Endocytosis, Metastasis and Beyond: Multiple Facets of SNX9.Trends in cell biology · 2017Review
- SopB-Mediated Recruitment of SNX18 FacilitatesFrontiers in cellular and infection microbiology · 2017Article
- BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission.Journal of cell science · 2016Article
- Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.Molecular & cellular proteomics : MCP · 2014Article
- Phosphorylation of the Bin, Amphiphysin, and RSV161/167 (BAR) domain of ACAP4 regulates membrane tubulation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation.PloS one · 2013Article
- Molecular basis for SNX-BAR-mediated assembly of distinct endosomal sorting tubules.The EMBO journal · 2012Article
- A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity.PloS one · 2012Article
- SNX12 role in endosome membrane transport.PloS one · 2012Article
- Expression of sorting nexin 18 (SNX18) is dynamically regulated in developing spinal motor neurons.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
- The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins.PloS one · 2010Article
- SNX-BAR proteins in phosphoinositide-mediated, tubular-based endosomal sorting.Seminars in cell & developmental biology · 2010Review
- WASP family proteins: Molecular mechanisms and implications in human disease.European journal of cell biologyArticle
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorting nexin 33 (SNX33) is a novel member of the sorting nexin superfamily with three predicted structural domains, SH3-PX-BAR. Very little is known about the cellular function of SNX33. In an effort to analyze its structure/function relationship, we attempted but failed to generate stable cell lines for short hairpin RNA or overexpression SNX33. Transient knockdown of SNX33 induces both HeLa and MCF7 cells to grow multiple long processes, delay the G(1)/M transition, and become more apoptotic, implying that SNX33 may control cell cycle process through influence the cytoskeleton. In vitro cell lineage analysis revealed that cells transfected with SNX33 failed to divide and became micronucleated, suggesting a specific defect in cytokinesis. Further analysis revealed that SNX33 induced the accumulation of actin at the perinuclear space, which might have disabled the cytokinetic machinery. However, SNX33 appears to mediate actin polymerization indirectly, as they do not interact with each other. SNX33 interacts with itself and SNX9. Interestingly, it also interacts with VCA domain of Wiskott-Aldrich syndrome protein (WASp), a protein known to be involved in actin polymerization. Indeed, cells overexpressing WASp failed to divide and form stable colonies as SNX33, consistent with the notion that SNX33 may interfere with cytokinesis. On the other hand, knockdown of WASp alleviates the phenotype induced by SNX33. Taken together, our results suggest that SNX33 plays a role in maintaining cell shape and cell cycle progression through its interaction with WASp.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.