ArticleProceedings of the National Academy of Sciences of the United States of America2009
Sorting in early endosomes reveals connections to docking- and fusion-associated factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
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- Crosstalk between autophagy inhibitors and endosome-related secretory pathways: a challenge for autophagy-based treatment of solid cancers.Molecular cancer · 2021Review
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- New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells.The Journal of cell biology · 2019Article
- Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.Scientific reports · 2019Article
- Reduction of aluminum ion neurotoxicity through a small peptide application - NAP treatment of Alzheimer's disease.Journal of food and drug analysis · 2019Article
- Alzheimer Aβ Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKα3 Neurons by Secretion.iScience · 2019Article
- Energetic cost of building a virus.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.Cell research · 2016Article
- VAMP8-dependent fusion of recycling endosomes with the plasma membrane facilitates T lymphocyte cytotoxicity.The Journal of cell biology · 2015Article
- ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.Journal of cell science · 2015Article
- Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.Nature communications · 2014Article
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- DNA-polymer conjugates for immune stimulation through Toll-like receptor 9 mediated pathways.Acta biomaterialia · 2014Article
- AKT facilitates EGFR trafficking and degradation by phosphorylating and activating PIKfyve.Science signaling · 2013Article
- Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Review
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Sorting of the FGF receptor 1 in a human glioma cell line.Histochemistry and cell biology · 2013Article
- Alternative splicing results in RET isoforms with distinct trafficking properties.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The early endosomes constitute a major sorting platform in eukaryotic cells. They receive material through fusion with endocytotic vesicles or with trafficking vesicles from the Golgi complex and later sort it into budding vesicles. While endosomal fusion is well understood, sorting is less characterized; the 2 processes are generally thought to be effected by different, unrelated machineries. We developed here a cell-free assay for sorting/budding from early endosomes, by taking advantage of their ability to segregate different cargoes (such as transferrin, cholera toxin subunit B, and low-density lipoprotein, LDL) into different carrier vesicles. Cargo separation required both carrier vesicle formation and active maturation of the endosomes. Sorting and budding were insensitive to reagents perturbing clathrin coats, coatomer protein complex-I (COPI) coats, dynamin, and actin, but were inhibited by anti-retromer subunit antibodies. In addition, the process required Rab-GTPases, phosphatidylinositol-3-phosphate, and, surprisingly, the docking factor early endosomal autoantigen 1 (EEA1). Sorting also required the function of the N-ethylmaleimide-sensitive factor (NSF), a well-known fusion cofactor, while it did not depend on preceding fusion of endosomes. We conclude that fusion, docking, and sorting/budding are interconnected at the molecular level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.