ArticleThe Journal of experimental medicine2009
Cholera toxin inhibits IL-12 production and CD8alpha+ dendritic cell differentiation by cAMP-mediated inhibition of IRF8 function.
Article in The Journal of experimental medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Pde1b controls cyclic nucleotide signaling in cDC1 and macrophages to regulate immune responses.EMBO reports · 2026Article
- Transamidation Down-Regulates Intestinal Immunity of Recombinant α-Gliadin in HLA-DQ8 Transgenic Mice.International journal of molecular sciences · 2021Article
- Malaria systems immunology: Plasmodium vivax induces tolerance during primary infection through dysregulation of neutrophils and dendritic cells.The Journal of infection · 2018Article
- Prostaglandin E2 Inhibition of IL-27 Production in Murine Dendritic Cells: A Novel Mechanism That Involves IRF1.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Nod2-mediated recognition of the microbiota is critical for mucosal adjuvant activity of cholera toxin.Nature medicine · 2016Article
- Differential Impact of LPG-and PG-Deficient Leishmania major Mutants on the Immune Response of Human Dendritic Cells.PLoS neglected tropical diseases · 2015Article
- Cholera toxin adjuvant promotes a balanced Th1/Th2/Th17 response independently of IL-12 and IL-17 by acting on Gsα in CD11b⁺ DCs.Mucosal immunology · 2015Article
- Mechanisms of Cholera Toxin in the Modulation of TH17 Responses.Critical reviews in immunology · 2015Review
- Helicobacter pylori vaccination: is there a path to protection?World journal of gastroenterology · 2014Review
- Human monocytes respond to extracellular cAMP through A2A and A2B adenosine receptors.Journal of leukocyte biology · 2014Article
- Cooperation of TLR12 and TLR11 in the IRF8-dependent IL-12 response to Toxoplasma gondii profilin.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Promotion of colonization and virulence by cholera toxin is dependent on neutrophils.Infection and immunity · 2013Article
- Francisella tularensis SchuS4 and SchuS4 lipids inhibit IL-12p40 in primary human dendritic cells by inhibition of IRF1 and IRF8.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Phosphodiesterase 4 inhibition in the treatment of psoriasis, psoriatic arthritis and other chronic inflammatory diseases.Dermatology and therapy · 2013Article
- Type II heat-labile enterotoxins: structure, function, and immunomodulatory properties.Veterinary immunology and immunopathology · 2013Review
- Cholera toxin enhances vaccine-induced protection against Mycobacterium tuberculosis challenge in mice.PloS one · 2013Article
- Innate immunostimulatory properties of allergens and their relevance to food allergy.Seminars in immunopathology · 2012Review
- Neutrophils are essential for containment of Vibrio cholerae to the intestine during the proinflammatory phase of infection.Infection and immunity · 2012Article
- Article
- Cholera toxin impairs the differentiation of monocytes into dendritic cells, inducing professional antigen-presenting myeloid cells.Infection and immunity · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 8 institutions in 3 countries.
Funding
Abstract
Prior studies have demonstrated that cholera toxin (CT) and other cAMP-inducing factors inhibit interleukin (IL)-12 production from monocytes and dendritic cells (DCs). We show that CT inhibits Th1 responses in vivo in mice infected with Toxoplasma gondii. This correlated with low serum IL-12 levels and a selective reduction in the numbers of CD8alpha(+) conventional DCs (cDCs) in lymphoid organs. CT inhibited the function of interferon (IFN) regulatory factor (IRF) 8, a transcription factor known to positively regulate IL-12p35 and p40 gene expression, and the differentiation of CD8alpha(+) and plasmacytoid DCs (pDCs). Fluorescence recovery after photobleaching analysis showed that exposure to CT, forskolin, or dibutyryl (db) cAMP blocked LPS and IFN-gamma-induced IRF8 binding to chromatin. Moreover, CT and dbcAMP inhibited the binding of IRF8 to the IFN-stimulated response element (ISRE)-like element in the mouse IL-12p40 promoter, likely by blocking the formation of ISRE-binding IRF1-IRF8 heterocomplexes. Furthermore, CT inhibited the differentiation of pDCs from fms-like tyrosine kinase 3 ligand-treated bone marrow cells in vitro. Therefore, because IRF8 is essential for IL-12 production and the differentiation of CD8alpha(+) cDCs and pDCs, these data suggest that CT and other Gs-protein agonists can affect IL-12 production and DC differentiation via a common mechanism involving IRF8.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.