ArticleNucleic acids research2009
Tracking transcription factor complexes on DNA using total internal reflectance fluorescence protein binding microarrays.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Article
- Affinity and competition for TBP are molecular determinants of gene expression noise.Nature communications · 2016Article
- Dry and wet approaches for genome-wide functional annotation of conventional and unconventional transcriptional activators.Computational and structural biotechnology journal · 2016Review
- Using protein-binding microarrays to study transcription factor specificity: homologs, isoforms and complexes.Briefings in functional genomics · 2015Review
- Absence of a simple code: how transcription factors read the genome.Trends in biochemical sciences · 2014Review
- The conformational state of the nucleosome entry-exit site modulates TATA box-specific TBP binding.Nucleic acids research · 2014Article
- Protein-DNA binding: complexities and multi-protein codes.Nucleic acids research · 2014Review
- STAT1:DNA sequence-dependent binding modulation by phosphorylation, protein:protein interactions and small-molecule inhibition.Nucleic acids research · 2013Article
- Single-molecule fluorescence resonance energy transfer shows uniformity in TATA binding protein-induced DNA bending and heterogeneity in bending kinetics.Biochemistry · 2012Article
- Eeny meeny miny moe, catch a transcript by the toe, or how to enumerate eukaryotic transcripts.Genes & development · 2012Article
- Assembly of the transcription machinery: ordered and stable, random and dynamic, or both?Chromosoma · 2011Review
- De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis.Nature biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a high-throughput protein binding microarray (PBM) assay to systematically investigate transcription regulatory protein complexes binding to DNA with varied specificity and affinity. Our approach is based on the novel coupling of total internal reflectance fluorescence (TIRF) spectroscopy, swellable hydrogel double-stranded DNA microarrays and dye-labeled regulatory proteins, making it possible to determine both equilibrium binding specificities and kinetic rates for multiple protein:DNA interactions in a single experiment. DNA specificities and affinities for the general transcription factors TBP, TFIIA and IIB determined by TIRF-PBM are similar to those determined by traditional methods, while simultaneous measurement of the factors in binary and ternary protein complexes reveals preferred binding combinations. TIRF-PBM provides a novel and extendible platform for multi-protein transcription factor investigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.