ArticleBiophysical journal2009
Diffusion and partitioning of fluorescent lipid probes in phospholipid monolayers.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Quantifying Small-Molecule Association with Lipid Membranes: Methods, Models, and Limitations.Membranes · 2026Review
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Measuring protein insertion areas in lipid monolayers by fluorescence correlation spectroscopy.Biophysical journal · 2021Article
- FCS Analysis of Protein Mobility on Lipid Monolayers.Biophysical journal · 2018Article
- Enabling Marangoni flow at air-liquid interfaces through deposition of aerosolized lipid dispersions.Journal of colloid and interface science · 2016Article
- Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets.Angewandte Chemie (International ed. in English) · 2016Article
- Measuring selective estrogen receptor modulator (SERM)-membrane interactions with second harmonic generation.Journal of the American Chemical Society · 2014Article
- Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure.Biophysical journal · 2013Article
- Single molecule probes of membrane structure: orientation of BODIPY probes in DPPC as a function of probe structure.The Analyst · 2012Article
- Atomistic simulation of lipid and DiI dynamics in membrane bilayers under tension.Physical chemistry chemical physics : PCCP · 2011Article
- Influence of lipid heterogeneity and phase behavior on phospholipase A2 action at the single molecule level.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pressure-dependent diffusion and partitioning of single lipid fluorophores in DMPC and DPPC monolayers were investigated with the use of a custom-made monolayer trough mounted on a combined fluorescence correlation spectroscopy (FCS) and wide-field microscopy setup. It is shown that lipid diffusion, which is essential for the function of biological membranes, is heavily influenced by the lateral pressure and phase of the lipid structure. Both of these may change dynamically during, e.g., protein adsorption and desorption processes. Using FCS, we measured lipid diffusion coefficients over a wide range of lateral pressures in DMPC monolayers and fitted them to a free-area model as well as the direct experimental observable mean molecular area. FCS measurements on DPPC monolayers were also performed below the onset of the phase transition (Pi < 5 mN/m). At higher pressures, FCS was not applicable for measuring diffusion coefficients in DPPC monolayers. Single-molecule fluorescence microscopy and differential scanning calorimetry clearly showed that this was due to heterogeneous partitioning of the lipid fluorophores in condensed phases. The results were compared with dye partitioning in giant lipid vesicles. These findings are significant in relation to the application of lipid fluorophores to study diffusion in both model systems and biological systems.
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