ArticleJournal of the American Chemical Society2009
Longitudinal-relaxation-enhanced NMR experiments for the study of nucleic acids in solution.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 109 citations in OpenAlex.
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- Application of NMR to Large RNAs.Journal of molecular biology · 2025Review
- Relaxation Optimized Heteronuclear Experiments for Extending the Size Limit of RNA Nuclear Magnetic Resonance.Journal of the American Chemical Society · 2025Article
- Imino chemical shift assignments of tRNABiomolecular NMR assignments · 2024Article
- Optimising in-cell NMR acquisition for nucleic acids.Journal of biomolecular NMR · 2024Article
- Targeting the Main Protease (MACS chemical biology · 2024Article
- PRESERVE: adding variable flip-angle excitation to transverse relaxation-optimized NMR spectroscopy.Magnetic resonance (Gottingen, Germany) · 2024Article
- Different modification pathways for m1A58 incorporation in yeast elongator and initiator tRNAs.Nucleic acids research · 2023Article
- E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation.Journal of biomolecular NMR · 2023Article
- Pressure pushes tRNAProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Structural Fingerprinting of Antisense Oligonucleotide Therapeutics by Solution NMR Spectroscopy.Pharmaceutical research · 2023Article
- Why are G-quadruplexes good at preventing protein aggregation?RNA biology · 2023Article
- Structural Fingerprinting of siRNA Therapeutics by Solution NMR Spectroscopy.Nucleic acid therapeutics · 2022Article
- Epitope-focused immunogen design based on the ebolavirus glycoprotein HR2-MPER region.PLoS pathogens · 2022Article
- NMR assignment of non-modified tRNABiomolecular NMR assignments · 2022Article
- Article
- A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1.International journal of molecular sciences · 2021Article
- Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics.Magnetic resonance (Gottingen, Germany) · 2021Review
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Atomic-resolution information on the structure and dynamics of nucleic acids is essential for a better understanding of the mechanistic basis of many cellular processes. NMR spectroscopy is a powerful method for studying the structure and dynamics of nucleic acids; however, solution NMR studies are currently limited to relatively small nucleic acids at high concentrations. Thus, technological and methodological improvements that increase the experimental sensitivity and spectral resolution of NMR spectroscopy are required for studies of larger nucleic acids or protein-nucleic acid complexes. Here we introduce a series of imino-proton-detected NMR experiments that yield an over 2-fold increase in sensitivity compared to conventional pulse schemes. These methods can be applied to the detection of base pair interactions, RNA-ligand titration experiments, measurement of residual dipolar (15)N-(1)H couplings, and direct measurements of conformational transitions. These NMR experiments employ longitudinal spin relaxation enhancement techniques that have proven useful in protein NMR spectroscopy. The performance of these new experiments is demonstrated for a 10 kDa TAR-TAR*(GA) RNA kissing complex and a 26 kDa tRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.