ArticleHistochemistry and cell biology2009
New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Protein Supplementation Does Not Affect Myogenic Adaptations to Resistance Training.Medicine and science in sports and exercise · 2017Trial
- Strength training increases the size of the satellite cell pool in type I and II fibres of chronically painful trapezius muscle in females.The Journal of physiology · 2011Trial
- Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2009Trial
- Desmin is not ubiquitously expressed in head and neck muscle fibers.Journal of anatomy · 2026Article
- Fast and slow myofiber nuclei, satellite cells, and size distribution with lifelong endurance exercise in men and women.Physiological reports · 2024Article
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- Lack ofeLife · 2022Article
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- Consistent expression pattern of myogenic regulatory factors in whole muscle and isolated human muscle satellite cells after eccentric contractions in humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- Desmin and dystrophin abnormalities in upper airway muscles of snorers and patients with sleep apnea.Respiratory research · 2019Article
- Coding Cell Identity of Human Skeletal Muscle Progenitor Cells Using Cell Surface Markers: Current Status and Remaining Challenges for Characterization and Isolation.Frontiers in cell and developmental biology · 2019Review
- Protein Availability and Satellite Cell Dynamics in Skeletal Muscle.Sports medicine (Auckland, N.Z.) · 2018Review
- Physiological Differences Between Low Versus High Skeletal Muscle Hypertrophic Responders to Resistance Exercise Training: Current Perspectives and Future Research Directions.Frontiers in physiology · 2018Review
- Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Resistance Training Enhances Skeletal Muscle Innervation Without Modifying the Number of Satellite Cells or their Myofiber Association in Obese Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2016Article
- Unique expression of cytoskeletal proteins in human soft palate muscles.Journal of anatomy · 2016Article
- Meeting the meat: delineating the molecular machinery of muscle development.Journal of animal science and technology · 2016Review
- Human Satellite Cell Transplantation and Regeneration from Diverse Skeletal Muscles.Stem cell reports · 2015Article
- Stem cell activation in skeletal muscle regeneration.Cellular and molecular life sciences : CMLS · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic substances and untrained subjects were re-examined. Significantly different results from those with staining for NCAM and nuclei were observed. There were three subtypes of SCs; NCAM(+)/Pax7(+) (94%), NCAM(+)/Pax7(-) (4%) and NCAM(-)/Pax7(+) (1%) but large individual variability existed. The proportion of SCs per nuclei within the basal lamina of myofibres (SC/N) was similar for all groups reflecting a balance between the number of SCs and myonuclei to maintain homeostasis. We emphasise that it is important to quantify both SC/N and the number of SCs per fibre. Our multiple marker method is more reliable for SC identification and quantification and can be used to evaluate other markers of muscle progenitor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.