ArticleJournal of bacteriology2009
Thioredoxin is involved in U(VI) and Cr(VI) reduction in Desulfovibrio desulfuricans G20.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Molecular Mechanisms Underlying Bacterial Uranium Resistance.Frontiers in microbiology · 2022Review
- Expression of Cytochrome c3 fromInternational journal of molecular sciences · 2021Article
- Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration.ISME communications · 2021Article
- Transcriptome Analysis Reveals Cr(VI) Adaptation Mechanisms inFrontiers in microbiology · 2021Article
- Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota.Communications biology · 2020Article
- Anion transport as a target of adaption to perchlorate in sulfate-reducing communities.The ISME journal · 2020Article
- New Plant Growth-Promoting, Chromium-DetoxifyingFrontiers in bioengineering and biotechnology · 2020Article
- Article
- Metalloid Reductase ofACS omega · 2018Article
- Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris.Applied microbiology and biotechnology · 2018Article
- Copper (II) binding of NAD(P)H- flavin oxidoreductase (NfoR) enhances its Cr (VI)-reducing ability.Scientific reports · 2017Article
- Complete genome sequence of the chromate-reducing bacterium Thermoanaerobacter thermohydrosulfuricus strain BSB-33.Standards in genomic sciences · 2015Article
- ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- Global transcriptome analysis of hexavalent chromium stress responses in Staphylococcus aureus LZ-01.Ecotoxicology (London, England) · 2014Article
- Article
- Genes required for alleviation of uranium toxicity in sulfate reducing bacterium Desulfovibrio alaskensis G20 [corrected].Ecotoxicology (London, England) · 2014Article
- Identification of Multiple Soluble Fe(III) Reductases in Gram-Positive Thermophilic Bacterium Thermoanaerobacter indiensis BSB-33.International journal of genomics · 2014Article
- Genome-wide transcriptional responses of two metal-tolerant symbiotic Mesorhizobium isolates to zinc and cadmium exposure.BMC genomics · 2013Article
- Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction.BMC microbiology · 2012Article
- Functional characterization of Crp/Fnr-type global transcriptional regulators in Desulfovibrio vulgaris Hildenborough.Applied and environmental microbiology · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transposon insertion mutant has been identified in a Desulfovibrio desulfuricans G20 mutant library that does not grow in the presence of 2 mM U(VI) in lactate-sulfate medium. This mutant has also been shown to be deficient in the ability to grow with 100 microM Cr(VI) and 20 mM As(V). Experiments with washed cells showed that this mutant had lost the ability to reduce U(VI) or Cr(VI), providing an explanation for the lower tolerance. A gene encoding a cyclic AMP (cAMP) receptor protein (CRP) was identified as the site of the transposon insertion. The remainder of the mre operon (metal reduction) contains genes encoding a thioredoxin, thioredoxin reductase, and an additional oxidoreductase whose substrate has not been predicted. Expression studies showed that in the mutant, the entire operon is downregulated, suggesting that the CRP may be involved in regulating expression of the whole operon. Exposure of the cells to U(VI) resulted in upregulation of the entire operon. CdCl(2), a specific inhibitor of thioredoxin activity, inhibits U(VI) reduction by washed cells and inhibits growth of cells in culture when U(VI) is present, confirming a role for thioredoxin in U(VI) reduction. The entire mre operon was cloned into Escherichia coli JM109 and the transformant developed increased U(VI) resistance and the ability to reduce U(VI) to U(IV). The oxidoreductase protein (MreG) from this operon was expressed and purified from E. coli. In the presence of thioredoxin, thioredoxin reductase, and NADPH, this protein was shown to reduce both U(VI) and Cr(VI), providing a mechanism for the cytoplasmic reduction of these metals.
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