ArticleJournal of bacteriology2009
Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Modified alginates for precision drug delivery: Advances in controlled-release and targeting systems.International journal of pharmaceutics: X · 2025Review
- Advances in alginate biosynthesis: regulation and production inFrontiers in bioengineering and biotechnology · 2025Review
- Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history.BMC genomics · 2024Article
- Analysis of unsaturated alginate oligosaccharides using high-performance anion exchange chromatography coupled with mass spectrometry.Analytical and bioanalytical chemistry · 2024Article
- Alginate-like polymers from full-scale aerobic granular sludge: content, recovery, characterization, and application for cadmium adsorption.Scientific reports · 2022Article
- Improved site-specific mutagenesis in Rhodococcus opacus using a novel conditional suicide plasmid.Applied microbiology and biotechnology · 2022Article
- Cloning and Characterization of a Novel Endo-Type Metal-Independent Alginate Lyase from the Marine BacteriaMarine drugs · 2022Article
- Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.Applied and environmental microbiology · 2022Article
- Genetic Regulation of Alginate Production inFrontiers in microbiology · 2022Review
- Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.Biotechnology for biofuels · 2021Review
- Increased c-di-GMP Levels Lead to the Production of Alginates of High Molecular Mass in Azotobacter vinelandii.Journal of bacteriology · 2020Article
- Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family.Scientific reports · 2020Article
- Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis inFrontiers in bioengineering and biotechnology · 2019Article
- Expression and Characterization of a New PolyG-Specific Alginate Lyase From Marine BacteriumFrontiers in microbiology · 2018Article
- Bacterial alginate production: an overview of its biosynthesis and potential industrial production.World journal of microbiology & biotechnology · 2017Review
- Different responses in the expression of alginases, alginate polymerase and acetylation genes during alginate production by Azotobacter vinelandii under oxygen-controlled conditions.Journal of industrial microbiology & biotechnology · 2017Article
- Structure and Polymannuronate Specificity of a Eukaryotic Member of Polysaccharide Lyase Family 14.The Journal of biological chemistry · 2017Article
- Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production.Iranian biomedical journal · 2017Article
- Biological function of a polysaccharide degrading enzyme in the periplasm.Scientific reports · 2016Article
- Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability.The Journal of biological chemistry · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alginates are polysaccharides composed of 1-4-linked beta-D-mannuronic acid and alpha-L-guluronic acid. The polymer can be degraded by alginate lyases, which cleave the polysaccharide using a beta-elimination reaction. Two such lyases have previously been identified in the soil bacterium Azotobacter vinelandii, as follows: the periplasmic AlgL and the secreted bifunctional mannuronan C-5 epimerase and alginate lyase AlgE7. In this work, we describe the properties of three new lyases from this bacterium, AlyA1, AlyA2, and AlyA3, all of which belong to the PL7 family of polysaccharide lyases. One of the enzymes, AlyA3, also contains a C-terminal module similar to those of proteins secreted by a type I secretion system, and its activity is stimulated by Ca(2+). All three enzymes preferably cleave the bond between guluronic acid and mannuronic acid, resulting in a guluronic acid residue at the new reducing end, but AlyA3 also degrades the other three possible bonds in alginate. Strains containing interrupted versions of alyA1, alyA3, and algE7 were constructed, and their phenotypes were analyzed. Genetically pure alyA2 mutants were not obtained, suggesting that this gene product may be important for the bacterium during vegetative growth. After centrifugation, cultures from the algE7 mutants form a large pellet containing alginate, indicating that AlgE7 is involved in the release of alginate from the cells. Upon encountering adverse growth conditions, A. vinelandii will form a resting stage called cyst. Alginate is a necessary part of the protective cyst coat, and we show here that strains lacking alyA3 germinate poorly compared to wild-type cells.
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