ArticleBiomaterials2009
The influence of RGD-bearing hydrogels on the re-expression of contractile vascular smooth muscle cell phenotype.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Biofunctionalized 3D-printed gelatin-alginate scaffolds with arginine-glycine-aspartic acid (RGD) peptides for enhancedJournal of dental sciences · 2026Article
- Design considerations for engineering 3D models to study vascular pathologies in vitro.Acta biomaterialia · 2021Review
- Crosslinking of Gelatin in Bicomponent Electrospun Fibers.Materials (Basel, Switzerland) · 2021Article
- Upregulation of Calcium Homeostasis Modulators in Contractile-To-Proliferative Phenotypical Transition of Pulmonary Arterial Smooth Muscle Cells.Frontiers in physiology · 2021Article
- Bioresorbable silk grafts for small diameter vascular tissue engineering applications: In vitro and in vivo functional analysis.Acta biomaterialia · 2020Article
- Biomimetic-engineered poly (ethylene glycol) hydrogel for smooth muscle cell migration.Tissue engineering. Part A · 2014Article
- The effect of pulsatile loading and scaffold structure for the generation of a medial equivalent tissue engineered vascular graft.BioResearch open access · 2013Article
- Characterization of corpus cavernosum smooth muscle cell phenotype in diabetic rats with erectile dysfunction.International journal of impotence research · 2012Article
- Endothelial cell attachment and shear response on biomimetic polymer-coated vascular grafts.Journal of biomedical materials research. Part A · 2012Article
- Endothelial Cell Vascular Smooth Muscle Cell Co-Culture Assay For High Throughput Screening Assays For Discovery of Anti-Angiogenesis Agents and Other Therapeutic Molecules.International journal of high throughput screening · 2010Article
- Molecular regulation of contractile smooth muscle cell phenotype: implications for vascular tissue engineering.Tissue engineering. Part B, Reviews · 2010Review
- Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering.Biomaterials · 2010Review
- The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype.Biomaterials · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
This study reports on the ability of poly(ethylene glycol) diacrylate (PEGDA) hydrogel scaffolds with pendant integrin-binding GRGDSP peptides (RGD-gels) to support the re-differentiation of cultured vascular smooth muscle cells (SMCs) toward a contractile phenotype. Human coronary artery SMCs were seeded on RGD-gels, hydrogels with other extracellular matrix derived peptides, fibronectin (FN) and laminin (LN). Differentiation was induced on RGD-gels with low serum medium containing soluble heparin, and the differentiation status was monitored by mRNA expression, protein expression, and intracellular protein organization of the contractile smooth muscle markers, smooth muscle alpha-actin, calponin, and SM-22alpha. RGD-gels supported a rapid induction (2.7- to 25-fold up-regulation) of SMC marker gene mRNA, with expression levels that were equivalent to FN and LN controls. Marker protein levels mirrored the changes in mRNA expression, with levels on RGD-gels indistinguishable from FN and LN controls. Furthermore, these markers co-localized in stress fibers within SMCs on RGD-gels suggesting the recapitulation of a contractile apparatus within the cells. These results indicate that SMCs cultured on RGD-bearing hydrogels can re-differentiate toward a contractile phenotype suggesting this material is an excellent candidate for further development as a bioactive scaffold that regulates SMC phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.