ArticleJournal of molecular biology2009
RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Brome mosaic virus detected in Kansas wheat co-infected with other common wheat viruses.Frontiers in plant science · 2023Article
- Residues located in the primase domain of the bacteriophage T7 primase-helicase are essential for loading the hexameric complex onto DNA.The Journal of biological chemistry · 2022Article
- Virus Mechanics under Molecular Crowding.The journal of physical chemistry. B · 2021Article
- Hsc70-2 is required for Beet black scorch virus infection through interaction with replication and capsid proteins.Scientific reports · 2018Article
- Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants.PLoS pathogens · 2017Article
- Coat Protein Regulation by CK2, CPIP, HSP70, and CHIP Is Required for Potato Virus A Replication and Coat Protein Accumulation.Journal of virology · 2017Article
- Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.Journal of virology · 2016Article
- Isolation and characterization of the DNA and protein binding activities of adenovirus core protein V.Journal of virology · 2014Article
- The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.Journal of molecular biology · 2014Article
- Two key arginine residues in the coat protein of Bamboo mosaic virus differentially affect the accumulation of viral genomic and subgenomic RNAs.Molecular plant pathology · 2014Article
- Comparative analysis of adeno-associated virus capsid stability and dynamics.Journal of virology · 2013Article
- N-terminal basic amino acid residues of Beet black scorch virus capsid protein play a critical role in virion assembly and systemic movement.Virology journal · 2013Article
- Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.Virus research · 2012Article
- Chemical reactivity of brome mosaic virus capsid protein.Journal of molecular biology · 2012Article
- RNA binding by the NS3 protease of the hepatitis C virus.Virus research · 2012Article
- The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter.RNA (New York, N.Y.) · 2012Article
- Depurination of Brome mosaic virus RNA3 inhibits its packaging into virus particles.Nucleic acids research · 2011Article
- The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein.Molecular plant pathology · 2011Review
- Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo.Virology · 2011Article
- A simple and general method for determining the protein and nucleic acid content of viruses by UV absorbance.Virology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Viral capsid proteins (CPs) can regulate gene expression and encapsulate viral RNAs. Low-level expression of the brome mosaic virus (BMV) CP was found to stimulate viral RNA accumulation, while higher levels inhibited translation and BMV RNA replication. Regulation of translation acts through an RNA element named the B box, which is also critical for the replicase assembly. The BMV CP has also been shown to preferentially bind to an RNA element named SLC that contains the core promoter for genomic minus-strand RNA synthesis. To further elucidate CP interaction with RNA, we used a reversible cross-linking-peptide fingerprinting assay to identify peptides in the capsid that contact the SLC, the B-box RNA, and the encapsidated RNA. Transient expression of three mutations made in residues within or close by the cross-linked peptides partially released the normal inhibition of viral RNA accumulation in agroinfiltrated Nicotiana benthamiana. Interestingly, two of the mutants, R142A and D148A, were found to retain the ability to down-regulate reporter RNA translation. These two mutants formed viral particles in inoculated leaves, but only R142A was able to move systemically in the inoculated plant. The R142A CP was found to have higher affinities for SLC and the B box compared with those of wild-type CP and to alter contacts to the RNA in the virion. These results better define how the BMV CP can interact with RNA and regulate different viral processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.