ArticleTransgenic research2009
Expression of rabies virus G protein in carrots (Daucus carota).
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Development of mRNA rabies vaccines.Human vaccines & immunotherapeutics · 2024Review
- Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation.Plant cell reports · 2022Article
- Plant-Derived Recombinant Vaccines against Zoonotic Viruses.Life (Basel, Switzerland) · 2022Review
- Transient Expression in Cytoplasm and Apoplast of Rotavirus VP6 Protein Fused to Anti-DEC205 Antibody in Nicotiana benthamiana and Nicotiana sylvestris.Molecular biotechnology · 2021Article
- New human rabies vaccines in the pipeline.Vaccine · 2019Review
- Recombinant Plant Engineering for Immunotherapeutic Production.Current molecular biology reports · 2017Review
- Plant-based oral vaccines against zoonotic and non-zoonotic diseases.Plant biotechnology journal · 2016Review
- Plant-based vaccines for animals and humans: recent advances in technology and clinical trials.Therapeutic advances in vaccines · 2015Review
- Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein.Iranian journal of biotechnology · 2015Article
- Carrot cells: a pioneering platform for biopharmaceuticals production.Molecular biotechnology · 2015Review
- State of research in the field of the creation of plant vaccines for veterinary use.Russian journal of plant physiology: a comprehensive Russian journal on modern phytophysiology · 2015Review
- The present and future of rabies vaccine in animals.Clinical and experimental vaccine research · 2013Article
- Expression and solubilization of insect cell-based rabies virus glycoprotein and assessment of its immunogenicity and protective efficacy in mice.Clinical and vaccine immunology : CVI · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antigens derived from various pathogens can readily be synthesized at high levels in plants in their authentic forms. Such antigens administered orally can induce an immune response and, in some cases, result in protection against a subsequent challenge. We here report the expression of rabies virus G protein into carrots. The G gene was subcloned into the pUCpSSrabG vector and then used to transform carrot embryogenic cells by particle bombardment. The carrot cells were selected in liquid medium, a method previously unreported. The presence of the transgene was verified by PCR, and by RT-PCR. By western blot, G protein transgene was identified in 93.3% of adult carrot roots. The G protein was quantified by densitometric analysis (range 0.4-1.2%). The expressed protein was antigenic in mice. This confirms that the carrot is an adequate system for antigen expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.