ArticlePloS one2009
Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.
- Systematic review: gastrointestinal infection and incident inflammatory bowel disease.Alimentary pharmacology & therapeutics · 2020Pooled it
- The zoonotic potential of Mycobacterium avium ssp. paratuberculosis: a systematic review and meta-analyses of the evidence.Epidemiology and infection · 2015Pooled it
- Antibiotic Therapy for Active Crohn's Disease Targeting Pathogens: An Overview and Update.Antibiotics (Basel, Switzerland) · 2024Review
- Selection of vaccine-candidate peptides fromFrontiers in immunology · 2024Article
- Review
- Quantitative Proteomics of Gut-Derived Th1 and Th1/Th17 Clones Reveal the Presence of CD28+ NKG2D- Th1 Cytotoxic CD4+ T cells.Molecular & cellular proteomics : MCP · 2016Article
- On deaf ears, Mycobacterium avium paratuberculosis in pathogenesis Crohn's and other diseases.World journal of gastroenterology · 2015Review
- Immune Responses to Intestinal Microbes in Inflammatory Bowel Diseases.Current allergy and asthma reports · 2015Review
- Pathogenesis of Crohn's disease: Bug or no bug.World journal of gastrointestinal pathophysiology · 2015Review
- Mycobacterium paratuberculosis as a cause of Crohn's disease.Expert review of gastroenterology & hepatology · 2015Review
- Dismicrobism in inflammatory bowel disease and colorectal cancer: changes in response of colocytes.World journal of gastroenterology · 2014Review
- The contribution of natural selection to present-day susceptibility to chronic inflammatory and autoimmune disease.Current opinion in immunology · 2014Review
- Mycobacterium avium subspecies paratuberculosis in the etiology of Crohn's disease, cause or epiphenomenon?World journal of gastroenterology · 2014Review
- Escherichia coli-host macrophage interactions in the pathogenesis of inflammatory bowel disease.World journal of gastroenterology · 2014Review
- Role of the gut microbiota in inflammatory bowel disease pathogenesis: what have we learnt in the past 10 years?World journal of gastroenterology · 2014Review
- Increased frequency of intestinal CD4+ T cells reactive with mycobacteria in patients with Crohn's disease.Scandinavian journal of gastroenterology · 2013Article
- Anti-inflammatory and antiapoptotic responses to infection: a common denominator of human and bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis.BioMed research international · 2013Review
- Mycobacterium avium subsp. paratuberculosis lipophilic antigen causes Crohn's disease-type necrotizing colitis in Mice.SpringerPlus · 2012Article
- Uptake and persistence of Mycobacterium avium subsp. paratuberculosis in human monocytes.Infection and immunity · 2012Article
- Mycobacterium avium subsp. paratuberculosis inhibits gamma interferon-induced signaling in bovine monocytes: insights into the cellular mechanisms of Johne's disease.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCrohn's disease (CD) is a chronic granulomatous inflammation of the intestine. The etiology is unknown, but an excessive immune response to bacteria in genetically susceptible individuals is probably involved. The response is characterized by a strong Th1/Th17 response, but the relative importance of the various bacteria is not known. METHODOLOGY/PRINCIPAL
findingsIn an attempt to address this issue, we made T-cell lines from intestinal biopsies of patients with CD (n = 11), ulcerative colitis (UC) (n = 13) and controls (n = 10). The T-cell lines were tested for responses to various bacteria. A majority of the CD patients with active disease had a dominant response to Mycobacterium avium subspecies paratuberculosis (MAP). The T cells from CD patients also showed higher proliferation in response to MAP compared to UC patients (p<0.025). MAP reactive CD4 T-cell clones (n = 28) were isolated from four CD patients. The T-cell clones produced IL-17 and/or IFN-gamma, while minimal amounts of IL-4 were detected. To further characterize the specificity, the responses to antigen preparations from different mycobacterial species were tested. One T-cell clone responded only to MAP and the very closely related M. avium subspecies avium (MAA) while another responded to MAP, MAA and Mycobacterium intracellulare. A more broadly reactive T-cell clone reacted to MAP1508 which belongs to the esx protein family. CONCLUSIONS/SIGNIFICANCE: The presence of MAP reactive T cells with a Th1 or Th1/Th17 phenotype may suggest a possible role of mycobacteria in the inflammation seen in CD. The isolation of intestinal T cells followed by characterization of their specificity is a valuable tool to study the relative importance of different bacteria in CD.
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