ArticleJournal of biomedicine & biotechnology2009
Improved coinfection with amphotropic pseudotyped retroviral vectors.
Article in Journal of biomedicine & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Optimized gene transduction in human lung organoids: A high-efficiency method for advanced research applications.Communications biology · 2025Article
- Cyclin-Dependent Kinase-Dependent Phosphorylation of Sox2 at Serine 39 Regulates Neurogenesis.Molecular and cellular biology · 2017Article
- Macrolides selectively inhibit mutant KCNJ5 potassium channels that cause aldosterone-producing adenoma.The Journal of clinical investigation · 2017Article
- Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts.Molecular medicine reports · 2017Article
- Overcoming barriers to reprogramming and differentiation in nonhuman primate induced pluripotent stem cells.Primate biology · 2017Article
- Mutated KCNJ5 activates the acute and chronic regulatory steps in aldosterone production.Journal of molecular endocrinology · 2016Article
- Induced Pluripotent Stem Cells from Nonhuman Primates.Methods in molecular biology (Clifton, N.J.) · 2016Article
- All roads lead to induced pluripotent stem cells: the technologies of iPSC generation.Stem cells and development · 2014Review
- Nonhuman primate induced pluripotent stem cells in regenerative medicine.Stem cells international · 2012Article
- Controlled gene expression in primary Lgr5 organoid cultures.Nature methods · 2011Article
- Generation of induced pluripotent stem cells from newborn marmoset skin fibroblasts.Stem cell research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Amphotropic pseudotyped retroviral vectors have typically been used to infect target cells without prior concentration. Although this can yield high rates of infection, higher rates may be needed where highly efficient coinfection of two or more vectors is needed. In this investigation we used amphotropic retroviral vectors produced by the Plat-A cell line and studied coinfection rates using green and red fluorescent proteins (EGFP and dsRed2). Target cells were primary human fibroblasts (PHF) and 3T3 cells. Unconcentrated vector preparations produced a coinfection rate of approximately 4% (defined as cells that are both red and green as a percentage of all cells infected). Optimized spinoculation, comprising centrifugation at 1200 g for 2 hours at 15 degrees C, increased the coinfection rate to approximately 10%. Concentration by centrifugation at 10,000 g or by flocculation using Polybrene increased the coinfection rate to approximately 25%. Combining the two processes, concentration by Polybrene flocculation and optimized spinoculation, increased the coinfection rate to 35% (3T3) or >50% (PHF). Improved coinfection should be valuable in protocols that require high transduction by combinations of two or more retroviral vectors.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.