ArticlePloS one2009
Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 222 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Heat-stable protein PGSL1 enhances pollen germination and tube growth at high temperature.Nature communications · 2025Article
- Establishing cell polarity in plants: the role of cytoskeletal structures and regulatory pathways.Frontiers in cell and developmental biology · 2025Review
- The polar code for patterning: how polarity and the cytoskeleton orchestrate asymmetric cell division during plant development.Frontiers in cell and developmental biology · 2025Review
- Imaging actin organisation and dynamics in 3D.Journal of cell science · 2024Article
- Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution.Plant communications · 2024Article
- Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.The Journal of cell biology · 2023Article
- Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Article
- Heterologous Expression of Full-Length and Truncated Human ZIP4 Zinc Transporter inBiomolecules · 2022Article
- RNAi-Mediated Knockdown of Calreticulin3a Impairs Pollen Tube Growth inInternational journal of molecular sciences · 2022Article
- Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Myosin XI drives polarized growth by vesicle focusing and local enrichment of F-actin in Physcomitrium patens.Plant physiology · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- A minus-end directed kinesin motor directs gravitropism in Physcomitrella patens.Nature communications · 2021Article
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes.The Plant cell · 2021Article
- Overexpression of Lifeact-GFP Disrupts F-Actin Organization in Cardiomyocytes and Impairs Cardiac Function.Frontiers in cell and developmental biology · 2021Article
- AtSEC22 Regulates Cell Morphogenesis via Affecting Cytoskeleton Organization and Stabilities.Frontiers in plant science · 2021Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundActin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments. METHODOLOGY/PRINCIPAL
findingsIn this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore. CONCLUSIONS/SIGNIFICANCE: Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.