ArticleArchaea (Vancouver, B.C.)2009
A Gateway platform for functional genomics in Haloferax volcanii: deletion of three tRNA modification genes.
Article in Archaea (Vancouver, B.C.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Review
- Cytidine Acetylation Across the Tree of Life.Accounts of chemical research · 2024Article
- Open Issues for Protein Function Assignment inGenes · 2021Article
- Haloarchaeal Carotenoids: Healthy Novel Compounds from Extreme Environments.Marine drugs · 2019Review
- Nucleotide resolution sequencing of N4-acetylcytidine in RNA.Methods in enzymology · 2019Article
- Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein.Scientific reports · 2018Article
- Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon.Nucleic acids research · 2016Article
- Deciphering the Translation Initiation Factor 5A Modification Pathway in Halophilic Archaea.Archaea (Vancouver, B.C.) · 2016Article
- Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate.ACS chemical biology · 2012Article
- Functional promiscuity of the COG0720 family.ACS chemical biology · 2012Article
- A 5-formyltetrahydrofolate cycloligase paralog from all domains of life: comparative genomic and experimental evidence for a cryptic role in thiamin metabolism.Functional & integrative genomics · 2011Article
- Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii.RNA (New York, N.Y.) · 2011Article
- Functional genomic and advanced genetic studies reveal novel insights into the metabolism, regulation, and biology of Haloferax volcanii.Archaea (Vancouver, B.C.) · 2011Article
- Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii.Archaea (Vancouver, B.C.) · 2010Article
- Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins in Haloferax volcanii.Applied and environmental microbiology · 2010Article
- Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP cyclohydrolase IB.Journal of bacteriology · 2009Article
- Transfer RNA modifications: nature's combinatorial chemistry playground.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In part due to the existence of simple methods for its cultivation and genetic manipulation, Haloferax volcanii is a major archaeal model organism. It is the only archaeon for which the whole set of post-transcriptionally modified tRNAs has been sequenced, allowing for an in silico prediction of all RNA modification genes present in the organism. One approach to check these predictions experimentally is via the construction of targeted gene deletion mutants. Toward this goal, an integrative "Gateway vector" that allows gene deletion in H. volcanii uracil auxotrophs was constructed. The vector was used to delete three predicted tRNA modification genes: HVO_2001 (encoding an archaeal transglycosyl tranferase or arcTGT), which is involved in archeosine biosynthesis; HVO_2348 (encoding a newly discovered GTP cyclohydrolase I), which catalyzes the first step common to archaeosine and folate biosynthesis; and HVO_2736 (encoding a member of the COG1444 family), which is involved in N(4)-acetylcytidine (ac(4)C) formation. Preliminary phenotypic analysis of the deletion mutants was conducted, and confirmed all three predictions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.