ArticleNature protocols2009
(13)C-based metabolic flux analysis.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 239 papers.
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Who cites it
239 citing papers in PubMed.
- Article
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- Trans-dimensional Bayesian model averaging for 13C-metabolic flux analysis: evidence-based flux inference under structural model uncertainty.Bioinformatics (Oxford, England) · 2026Article
- Atomic elementary flux modes explain the steady state flow of metabolites in large-scale flux networks.Cell reports methods · 2026Article
- Microbe-dependent inter-organ communication regulates germline stem cell proliferation inbioRxiv : the preprint server for biology · 2026Article
- Metabolic Flux Analysis Reveals Entner-Doudoroff Pathway Dominance in Heterotrophic Deep-Sea Bacterial Isolates.Environmental microbiology reports · 2026Article
- Beyond Correlation: Constraint Architecture Explains Proteome-Metabolome Decoupling.International journal of molecular sciences · 2026Review
- Modeling lipid homeostasis using stable isotope tracing and flux analysis.Cell metabolism · 2026Article
- Evaluating transcriptomic integration for cyanobacterial constraint-based metabolic modelling.Frontiers in bioinformatics · 2026Article
- 13CFLUX - third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis.Bioinformatics (Oxford, England) · 2025Article
- Cross-kingdom mechanisms of trained immunity in plant systemic acquired resistance.Nature plants · 2025Review
- PositionalThe New phytologist · 2025Article
- Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides.Microbial biotechnology · 2025Article
- Article
- Metabolites are not genes - avoiding the misuse of pathway analysis in metabolomics.Nature metabolism · 2025Article
- An organism-level quantitative flux model of energy metabolism in mice.Cell metabolism · 2025Article
- Dissecting apicoplast functions through continuous cultivation of Toxoplasma gondii devoid of the organelle.Nature communications · 2025Article
- Review
- A Guide to Metabolic Network Modeling for Plant Biology.Plants (Basel, Switzerland) · 2025Review
179 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable isotope, and in particular (13)C-based flux analysis, is the exclusive approach to experimentally quantify the integrated responses of metabolic networks. Here we describe a protocol that is based on growing microbes on (13)C-labeled glucose and subsequent gas chromatography mass spectrometric detection of (13)C-patterns in protein-bound amino acids. Relying on publicly available software packages, we then describe two complementary mathematical approaches to estimate either local ratios of converging fluxes or absolute fluxes through different pathways. As amino acids in cell protein are abundant and stable, this protocol requires a minimum of equipment and analytical expertise. Most other flux methods are variants of the principles presented here. A true alternative is the analytically more demanding dynamic flux analysis that relies on (13)C-pattern in free intracellular metabolites. The presented protocols take 5-10 d, have been used extensively in the past decade and are exemplified here for the central metabolism of Escherichia coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.