ArticleThe American journal of pathology2009
Paradoxical effects of PDGF-BB overexpression in endothelial cells on engineered blood vessels in vivo.
Article in The American journal of pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling.Proceedings of the National Academy of Sciences of the United States of America · 2014Trial
- Regulation of PDGF-BB signaling in placental pericytes by soluble PDGFRβ isoforms: implications for fetoplacental vascular development.Angiogenesis · 2026Article
- Autologous and synthetic vascular grafts trigger different host responses in the anastomotic regions and in the perivascular adipose tissue during the early healing phase.Materials today. Bio · 2025Article
- Salivary chemokines and growth factors in patients with ischemic stroke.Scientific reports · 2025Article
- CCL7 as a novel inflammatory mediator in cardiovascular disease, diabetes mellitus, and kidney disease.Cardiovascular diabetology · 2022Review
- Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.Gels (Basel, Switzerland) · 2022Review
- 3D tumor angiogenesis models: recent advances and challenges.Journal of cancer research and clinical oncology · 2021Review
- Platelet-derived growth factor beta is a potent inflammatory driver in paediatric high-grade glioma.Brain : a journal of neurology · 2021Article
- Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture.International journal of molecular sciences · 2021Article
- Cell seeding accelerates the vascularization of tissue engineering constructs in hypertensive mice.Hypertension research : official journal of the Japanese Society of Hypertension · 2021Article
- Lupus serum induces inflammatory interaction with neutrophils in human glomerular endothelial cells.Lupus science & medicine · 2020Article
- Tissue Engineering Using Vascular Organoids From Human Pluripotent Stem Cell Derived Mural Cell Phenotypes.Frontiers in bioengineering and biotechnology · 2020Article
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- Horizontal alignment of 5' -> 3' intergene distance segment tropy with respect to the gene as the conserved basis for DNA transcription.Future science OA · 2017Article
- Pressuromodulation at the cell membrane as the basis for small molecule hormone and peptide regulation of cellular and nuclear function.Journal of translational medicine · 2015Article
- Developmental and tumoral vascularization is regulated by G protein-coupled receptor kinase 2.The Journal of clinical investigation · 2013Article
- Dissecting the role of human embryonic stem cell-derived mesenchymal cells in human umbilical vein endothelial cell network stabilization in three-dimensional environments.Tissue engineering. Part A · 2013Article
- Pericyte-derived MFG-E8 regulates pathologic angiogenesis.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- Cell-surface sensors for real-time probing of cellular environments.Nature nanotechnology · 2011Article
- Spontaneous nonthymic tumors in SCID mice.Comparative medicine · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Therapeutic revascularization with either exogenous angiogenic growth factors or vascular cells has yet to demonstrate efficacy in the clinic. Injection of angiogenic growth factors often produces unstable and abnormal blood vessels. Blood vascular networks derived from implanted endothelial cells persist only transiently due to the insufficient recruitment of perivascular cells. We hypothesize that a combination of the two approaches may act synergistically to yield a better result. To enhance the recruitment of perivascular cells, human umbilical vein endothelial cells were genetically modified to overexpress platelet-derived growth factor (PDGF)-BB. PDGF-BB overexpression promoted both proliferation and migration of perivascular precursor cells (10T1/2 cells) in vitro. When mock-infected endothelial cells were implanted alone in vivo, they formed transient blood vascular networks that regressed by day 30. PDGF-BB overexpression enhanced the survival of endothelial cells in vivo. However, the PDGF-BB-expressing vessel network failed to establish patent blood flow. Co-implantation of PDGF-BB-overexpressing endothelial cells with 10T1/2 cells paradoxically resulted in the rapid regression of the vascular networks in vivo. PDGF-BB stimulated the expression of both chemokine (C-C motif) ligand 2 (CCL2) and CCL7 in 10T1/2 cells and led to the increased accumulation of macrophages in vivo. These results suggest a potential negative interaction between angiogenic growth factors and vascular cells; their use in combination should be carefully tested in vivo for such opposing effects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.