ArticleMolecular biology of the cell2009
Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Structure of a myosin•adaptor complex and pairing by cargo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RNA imaging in living cells - methods and applications.RNA biology · 2014Review
- In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation.The Journal of cell biology · 2013Article
- Single-molecule reconstitution of mRNA transport by a class V myosin.Nature structural & molecular biology · 2013Article
- Principles of mRNA transport in yeast.Cellular and molecular life sciences : CMLS · 2012Review
- Inducible control of subcellular RNA localization using a synthetic protein-RNA aptamer interaction.PloS one · 2012Article
- Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.The Journal of cell biology · 2011Article
- The role of actin and myosin during spermatogenesis.Molecular biology reports · 2011Review
- Molecular motors: directing traffic during RNA localization.Critical reviews in biochemistry and molecular biology · 2011Review
- Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.The Journal of cell biology · 2010Article
- The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.The Journal of cell biology · 2010Article
- The path to visualization of walking myosin V by high-speed atomic force microscopy.Biophysical reviewsArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Myo4p, a single-headed and nonprocessive class V myosin in budding yeast, transports >20 different mRNAs asymmetrically to the bud. Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter cell, a process that also requires the adapter protein She3p and the dimeric mRNA-binding protein She2p. The rod region of Myo4p, but not the globular tail, is essential for correct localization of ASH1 mRNA, confirming that the rod contains the primary binding site for She3p. The requirement for both the rod region and She3p can be bypassed by directly coupling the mRNA-binding protein She2p to Myo4p. ASH1 mRNA was also correctly localized when one motor was bound per dimeric She2p, or when two motors were joined together by a leucine zipper. Because multiple mRNAs are cotransported to the bud, it is likely that this process involves multiple motor transport regardless of the number of motors per zip code. Our results show that the most important feature for correct localization is the retention of coupling between all the members of the complex (Myo4p-She3p-She2p-ASH1 mRNA), which is aided by She3p being a tightly bound subunit of Myo4p.
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