ArticleThe Journal of allergy and clinical immunology2009
Expression of functional leukotriene B4 receptors on human airway smooth muscle cells.
Article in The Journal of allergy and clinical immunology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Redox-sensitive GPCR signaling drives Gq-dependent CaAmerican journal of physiology. Cell physiology · 2026Article
- CD Molecules Nomenclature 2025: Antibody Validation and Expression Profiling of Immune System G Protein-Coupled Receptors.European journal of immunology · 2025Article
- Neutrophils and Asthma.Diagnostics (Basel, Switzerland) · 2022Review
- Leukotriene BHeliyon · 2021Article
- Roles of airway smooth muscle dysfunction in chronic obstructive pulmonary disease.Journal of translational medicine · 2018Review
- MicroRNA-638 inhibits human airway smooth muscle cell proliferation and migration through targeting cyclin D1 and NOR1.Journal of cellular physiology · 2018Review
- Regulation of human airway smooth muscle cell migration and relevance to asthma.Respiratory research · 2017Review
- Cigarette smoke-induced lung inflammation in COPD mediated via LTB4/BLT1/SOCS1 pathway.International journal of chronic obstructive pulmonary disease · 2016Article
- Human articular chondrocytes express functional leukotriene B4 receptors.Journal of anatomy · 2015Article
- Leukotriene B4 receptor locus gene characterisation and association studies in asthma.BMC medical genetics · 2012Article
- Profiling of differentially expressed genes using suppression subtractive hybridization in an equine model of chronic asthma.PloS one · 2012Article
- Motility, survival, and proliferation.Comprehensive Physiology · 2012Review
- Leukotrienes and airway inflammation.Biochimica et biophysica acta · 2011Review
- Direct evidence for functional smooth muscle myosin II in the 10S self-inhibited monomeric conformation in airway smooth muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Pharmacological inhibition of BLT1 diminishes early abdominal aneurysm formation.Atherosclerosis · 2010Article
- An update on the role of leukotrienes in asthma.Current opinion in allergy and clinical immunology · 2010Review
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundLeukotriene B4 (LTB4) increases in induced sputum and exhaled breath condensate in people with asthma. Furthermore, the T(H)2-type immune response and airway hyperresponsiveness induced by ovalbumin sensitization is markedly suppressed in LTB4 receptor (BLT) 1 null mice. These studies suggest that LTB4 may contribute to asthma pathophysiology. However, the direct effects of LTB4 on human airway smooth muscle (ASM) have not been studied.
objectivesWe sought to determine the expression of LTB4 receptors on human ASM and its functional role in mediating responses of human ASM cells, and the effect of LTB4 on these cells.
methodsImmunohistochemistry, RT-PCR, Western blotting, and flow cytometry were used to determine the expression of LTB4 receptors. To determine the effect of LTB4 on human ASM cells, cell proliferation was assessed by counting cells, and chemokinesis was assessed by gold particle phagokinesis assay.
resultsWe confirmed expression of both BLT1 and BLT2 in human ASM cells in bronchial tissue and in cell culture. LTB4 markedly induced cyclin D1 expression, proliferation, and chemokinesis of human ASM cells. LTB4 also induced phosphorylation of both p42/p44 mitogen-activated protein kinase (MAPK) and downstream PI3 kinase effector, Akt1. However, we observed no induction of c-Jun N-terminal kinase or p38 MAPK. Notably, LTB4-induced migration and proliferation of ASM cells were inhibited by the BLT1 specific antagonist, U75302, and by inhibitors of p42/p44 MAPK phosphorylation (U1026), and PI3 kinase (LY294002).
conclusionsThese observations are the first to suggest a role for a LTB4-BLT1 signaling axis in ASM responses that may contribute to the pathogenesis of airway remodeling in asthma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.