ArticlePlasmid2009
New yeast recombineering tools for bacteria.
Article in Plasmid, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 104 citations in OpenAlex.
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- Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.Communications biology · 2025Article
- Generation and comparison of broad-host range inducible expression vectors for use in Gram-negative bacteria including ESKAPE pathogens.Proceedings of the West Virginia Academy of Science · 2025Article
- The pGinger Family of Expression Plasmids.Microbiology spectrum · 2023Article
- Genetic Manipulation of Corynebacterium mastitidis to Better Understand the Ocular Microbiome.Investigative ophthalmology & visual science · 2023Article
- A rise in the frequency ofFrontiers in cellular and infection microbiology · 2023Article
- A Functional Analysis of the Purine Salvage Pathway in Acetobacter fabarum.Journal of bacteriology · 2022Article
- Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Applied and environmental microbiology · 2022Article
- Development of dual-inducible duet-expression vectors for tunable gene expression control and CRISPR interference-based gene repression in Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
- Antibiotics Used in Empiric Treatment of Ocular Infections Trigger the Bacterial Rcs Stress Response System Independent of Antibiotic Susceptibility.Antibiotics (Basel, Switzerland) · 2021Article
- The Rcs Stress Response System Regulator GumB Modulates Serratia marcescens-Induced Inflammation and Bacterial Proliferation in a Rabbit Keratitis Model and CytotoxicityInfection and immunity · 2021Article
- Differential susceptibility of airway and ocular surface cell lines to FlhDC-mediated virulence factors PhlA and ShlA fromJournal of medical microbiology · 2021Article
- Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Applied and environmental microbiology · 2020Article
- Xylose-Inducible Promoter Tools forApplied and environmental microbiology · 2020Article
- Leveraging host metabolism for bisdemethoxycurcumin production inMetabolic engineering communications · 2020Article
- Omics-driven identification and elimination of valerolactam catabolism inMetabolic engineering communications · 2019Article
- Use of Collagen Binding Domains to Deliver Molecules to the Cornea.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2019Article
- Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria.Nature communications · 2019Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Recombineering with Saccharomyces cerevisiae is a powerful methodology that can be used to clone multiple unmarked pieces of DNA to generate complex constructs with high efficiency. Here, we introduce two new tools that utilize the native recombination enzymes of S. cerevisiae to facilitate the manipulation of DNA. First, yeast recombineering was used to make directed nested deletions in a bacteria-yeast shuttle plasmid using only one or two single stranded oligomers, thus obviating the need for a PCR step. Second, we have generated several new shuttle vectors for yeast recombineering capable of replication in a wide variety of bacterial genera. As a demonstration of utility, some of the approaches and vectors generated in this study were used to make a pigP deletion mutation in the opportunistic pathogen Serratia marcescens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.