Evidence map›Paper›PMID 19476714›Full record

ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2009

A PCR-based strategy for detection of mouse parvovirus.

James D Macy, Frank X Paturzo, Lisa J Ball-Goodrich, Susan R Compton

Open access · greenAbstract read
In one paragraph

Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 20 citations in OpenAlex.

  1. Confirmation of Pathogen 'Burnout' in Mouse Colonies with Previous Evidence of Infection with Parvovirus and Rotavirus.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022
    Article
  2. Evaluation of In-cage Filter Paper as a Replacement for Sentinel Mice in the Detection of Murine Pathogens.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021
    Article
  3. PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020
    Article
  4. Article
  5. False-positive results after environmental pinworm PCR testing due to Rhabditid nematodes in Corncob bedding.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014
    Article
  6. Effect of immunodeficiency on MPV shedding and transmission.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013
    Article
  7. Maternal antibodies or nonproductive infections confound the need for rederivation.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013
    Article
  8. Unsterilized feed as the apparent cause of a mouse parvovirus outbreak.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013
    Article
  9. Transmission of mouse parvovirus to neonatal mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2012
    Article
  10. Transmission of mouse parvovirus by fomites.Journal of the American Association for Laboratory Animal Science : JAALAS · 2012
    Article
  11. Detection and control of mouse parvovirus.Journal of the American Association for Laboratory Animal Science : JAALAS · 2011
    Article
  12. Effect of murine norovirus infection on mouse parvovirus infection.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010
    Article
  13. The rodent quarantine quagmire.Journal of the American Association for Laboratory Animal Science : JAALAS · 2009
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

James D MacyYale Animal Resources Center, Yale University School of Medicine, New Haven, Connecticut, USA. james.macy@yale.edu
Frank X Paturzo
Lisa J Ball-Goodrich
Susan R Compton
Yale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mouse parvovirus (MPV) infection is difficult to address because it is asymptomatic, persists for as long as 9 wk, and occurs in small subpopulations of mice. The efficacy of a PCR-based cage swabbing strategy for MPV detection was tested. On postinoculation days (PID) 3 through 63, feces were collected from MPV-infected SW mice or the wire bars and cage wall above and below the bedding were swabbed. MPV DNA was detected in all cages in all locations on PID 7 and 14 but only below the bedding on PID 21. Swabbing below the bedding detected MPV in most cages through PID 42. Sentinels exposed to soiled cages on PID 7 and 14 but not on PID 21 seroconverted. MPV was detected in feces from all cages until PID 33 and in at least 1 cage until PID 56. In BALB/c mice, MPV was detected in feces and on cage swabs on PID 5 to 14, and 80% of sentinels exposed to soiled cages on PID 7 and 14 seroconverted. In comparison, MPV infection of C57BL/6 mice was detected in feces on PID 5 to 14 and on swabs on PID 5 and 7, and 30% of sentinels exposed to soiled cages on PID 7 and 14 seroconverted. Swabbing of multiple cages in rows in which only 1 cage contained MPV-infected mice was ineffective. In conclusion, swabbing of individual cages can be used in a genotype-dependent manner as an adjunct to soiled bedding sentinels during the first 2 wk of infection.

Indexed as

AnimalsFecesGenotypeHousing, AnimalMiceParvovirusPolymerase Chain Reaction

Identifiers

PMID19476714
PMCPMC2696828
OpenAlexW160885453

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.