ArticleBMC microbiology2009
A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Virulence Profiling of Multidrug-Resistant ClinicalMicrobial drug resistance (Larchmont, N.Y.) · 2026Article
- Plasmid diversity, antibiotic resistance and virulence genes associated with Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa.World journal of microbiology & biotechnology · 2026Article
- Development and validation of a hierarchical machine learning method using MALDI-TOF mass spectrometry for rapid SCCmec typing and PVL detection in MRSA: a multi-centre study.Emerging microbes & infections · 2025Article
- Isolation and encapsulation of bacteriophage with chitosan nanoparticles for biocontrol of multidrug-resistant methicillin-resistant Staphylococcus aureus isolated from broiler poultry farms.Scientific reports · 2024Article
- A report of coagulase-negativeIranian journal of veterinary research · 2024Article
- Detection ofFrontiers in immunology · 2024Article
- The Occurrence and Characteristics of Methicillin-Resistant Staphylococcal Isolates from Foods and Containers.Antibiotics (Basel, Switzerland) · 2023Article
- Development of diagnostic tests for pathogen identification and detection of antimicrobial resistance on WHO global priority pathogens using modular real-time nucleic acid amplification test.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Molecular Characterization of Methicillin-Resistant Staphylococci from the Dairy Value Chain in Two Indian States.Pathogens (Basel, Switzerland) · 2023Article
- Prevalence and epidemiological characteristics of methicillin-resistantAccess microbiology · 2023Article
- Pre-operative Colonization byRevista brasileira de ortopedia · 2022Article
- Prevalence of S. aureus and/or MRSA from seafood products from Indian seafood products.BMC microbiology · 2022Article
- Evaluation of in-house cefoxitin screening broth to determine methicillin-resistant staphylococci.Heliyon · 2022Article
- Virulence and antimicrobial resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle.PloS one · 2022Article
- Draft Genome Sequence of Methicillin-Resistant Staphylococcus aureus Harboring Staphylococcal Cassette ChromosomeMicrobiology resource announcements · 2021Article
- Whole genome sequence analysis of multi drug resistant community associated methicillin resistantPeerJ · 2021Article
- Acquisition Risk Factors of the SCCAntibiotics (Basel, Switzerland) · 2020Article
- Molecular characterisation of methicillin-resistantEpidemiology and infection · 2020Article
- Molecular detection and typing of methicillin-resistantVeterinary world · 2019Article
- Molecular and phylogenetic study ofVeterinary world · 2019Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaphylococcus aureus is a major human pathogen, especially methicillin-resistant S. aureus (MRSA), which causes a wide range of hospital and community-acquired infections worldwide. Conventional testing for detection of MRSA takes 2-5 days to yield complete information of the organism and its antibiotic sensitivity pattern.
resultsThe present study focused on the development of a pentaplex PCR assay for the rapid detection of MRSA. The assay simultaneously detected five genes, namely 16S rRNA of the Staphylococcus genus, femA of S. aureus, mecA that encodes methicillin resistance, lukS that encodes production of Panton-Valentine leukocidin (PVL), a necrotizing cytotoxin, and one internal control. Specific primer pairs were successfully designed and simultaneously amplified the targeted genes. The analytical sensitivity and specificity of the pentaplex PCR assay was evaluated by comparing it with the conventional method. The analytical sensitivity of the pentaplex PCR at the DNA level was found to be 10 ng DNA. The analytical specificity was evaluated with 34 reference staphylococci and non-staphylococcal strains and was found to be 100%. The diagnostic evaluation of MRSA carried out using 230 clinical isolates, showed 97.6% of sensitivity, 99.3% of specificity, 98.8% of positive predictive value and 98.6% of negative predictive value compared to the conventional method. The presence of an internal control in the pentaplex PCR assay is important to exclude false-negative cases.
conclusionThe pentaplex PCR assay developed was rapid and gave results within 4 h, which is essential for the identification of Staphylococcus spp., virulence and their resistance to methicillin. Our PCR assay may be used as an effective surveillance tool to survey the prevalence of MRSA and PVL-producing strains in hospitals and the community.
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