ArticleProteome science2009
Mitochondrial proteomics on human fibroblasts for identification of metabolic imbalance and cellular stress.
Article in Proteome science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Energy metabolism alteration and gene expression reprogramming in a cell model of high fat load non-alcoholic fatty liver disease.BMC gastroenterology · 2026Article
- Remodelling of the Mitochondrial Bioenergetic Pathways in Human Cultured Fibroblasts with Carbohydrates.Biology · 2023Article
- Integrated analysis of proteome and transcriptome changes in the mucopolysaccharidosis type VII mouse hippocampus.Molecular genetics and metabolism · 2016Article
- Application of an Image Cytometry Protocol for Cellular and Mitochondrial Phenotyping on Fibroblasts from Patients with Inherited Disorders.JIMD reports · 2016Article
- Selected reaction monitoring as an effective method for reliable quantification of disease-associated proteins in maple syrup urine disease.Molecular genetics & genomic medicine · 2014Article
- Modulation of mitochondrial bioenergetics in a skeletal muscle cell line model of mitochondrial toxicity.Redox biology · 2014Article
- MitProNet: A knowledgebase and analysis platform of proteome, interactome and diseases for mammalian mitochondria.PloS one · 2014Article
- Low glucose but not galactose enhances oxidative mitochondrial metabolism in C2C12 myoblasts and myotubes.PloS one · 2013Article
- Fragile X syndrome: a pilot proton magnetic resonance spectroscopy study in premutation carriers.Journal of neurodevelopmental disorders · 2012Article
- Mitochondrial proteomics--a tool for the study of metabolic disorders.Journal of inherited metabolic disease · 2012Review
- Analysis of the mitochondrial proteome in multiple sclerosis cortex.Biochimica et biophysica acta · 2011Article
- Toxic response caused by a misfolding variant of the mitochondrial protein short-chain acyl-CoA dehydrogenase.Journal of inherited metabolic disease · 2011Article
- Article
- Influence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.).Proteome science · 2010Article
- Lipogenesis is decreased by grape seed proanthocyanidins according to liver proteomics of rats fed a high fat diet.Molecular & cellular proteomics : MCP · 2010Article
- Antioxidant dysfunction: potential risk for neurotoxicity in ethylmalonic aciduria.Journal of inherited metabolic disease · 2010Article
- Transcriptome response to pollutants and insecticides in the dengue vector Aedes aegypti using next-generation sequencing technology.BMC genomics · 2010Article
- The mitochondrial proteome and human disease.Annual review of genomics and human genetics · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMitochondrial proteins are central to various metabolic activities and are key regulators of apoptosis. Disturbance of mitochondrial proteins is therefore often associated with disease. Large scale protein data are required to capture the mitochondrial protein levels and mass spectrometry based proteomics is suitable for generating such data. To study the relative quantities of mitochondrial proteins in cells from cultivated human skin fibroblasts we applied a proteomic method based on nanoLC-MS/MS analysis of iTRAQ-labeled peptides.
resultsWhen fibroblast cultures were exposed to mild metabolic stress - by cultivation in galactose medium- the amount of mitochondria appeared to be maintained whereas the levels of individual proteins were altered. Proteins of respiratory chain complex I and IV were increased together with NAD+-dependent isocitrate dehydrogenase of the citric acid cycle illustrating cellular strategies to cope with altered energy metabolism. Furthermore, quantitative protein data, with a median standard error below 6%, were obtained for the following mitochondrial pathways: fatty acid oxidation, citric acid cycle, respiratory chain, antioxidant systems, amino acid metabolism, mitochondrial translation, protein quality control, mitochondrial morphology and apoptosis.
conclusionThe robust analytical platform in combination with a well-defined compendium of mitochondrial proteins allowed quantification of single proteins as well as mapping of entire pathways. This enabled characterization of the interplay between metabolism and stress response in human cells exposed to mild stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.