ArticleJournal of medical virology2009
A real-time PCR assay to identify and discriminate among wild-type and vaccine strains of varicella-zoster virus and herpes simplex virus in clinical specimens, and comparison with the clinical diagnoses.
Article in Journal of medical virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00007501 (CSP #403 - Trial of Varicella Zoster Vaccine for the Prevention of Herpes Zoster and Its Complications), which is not on this map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
CSP #403 - Trial of Varicella Zoster Vaccine for the Prevention of Herpes Zoster and Its Complications
Who cites it
31 citing papers in PubMed, 71 citations in OpenAlex.
- Varicella-zoster virus-specific antibody responses in 50-59-year-old recipients of zoster vaccine.The Journal of infectious diseases · 2013Trial
- Persistence of the efficacy of zoster vaccine in the shingles prevention study and the short-term persistence substudy.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Trial
- Efficacy, safety, and tolerability of herpes zoster vaccine in persons aged 50-59 years.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Trial
- Article
- When two truths collide: overlapping herpes zoster and leukocytoclastic vasculitis in an atypical presentation.Diagnosis (Berlin, Germany) · 2025Article
- Status of Herpes Zoster and Herpes Zoster Vaccines in 2023: A position paper.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2023Review
- Towards a Rapid-Turnaround Low-Depth Unbiased Metagenomics Sequencing Workflow on the Illumina Platforms.Bioengineering (Basel, Switzerland) · 2023Article
- First detection of Varicella Zoster Virus clade 9 cases in India during mpox surveillance.Annals of medicine · 2023Article
- Multicenter Evaluation of the Simplexa VZV Direct Assay for Detection of Varicella-Zoster Virus in Cerebrospinal Fluid and Lesion-Swab Specimens.Journal of clinical microbiology · 2022Article
- Stroke-like Symptoms as Presenting Signs of Varicella Zoster Meningitis in an Immunocompetent Adult.Cureus · 2022Article
- Research Trends and Hotspots on Herpes Zoster: A 10-Year Bibliometric Analysis (2012-2021).Frontiers in medicine · 2022Article
- Varicella-zoster and herpes simplex virus reactivation post-COVID-19 vaccination: a review of 40 cases in an International Dermatology Registry.Journal of the European Academy of Dermatology and Venereology : JEADV · 2022Article
- Rare neurological complication in an adolescent with kidney failure secondary to systemic lupus erythematosus: Answers.Pediatric nephrology (Berlin, Germany) · 2021Article
- Rare neurological complication in an adolescent with kidney failure secondary to systemic lupus erythematosus: Questions.Pediatric nephrology (Berlin, Germany) · 2021Article
- Article
- Varicella Zoster Virus Encephalitis.Clinical practice and cases in emergency medicine · 2019Article
- Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704Research in pharmaceutical sciences · 2019Article
- Recurrent strokes, central nervous system vasculitis, and acquired protein S deficiency secondary to varicella zoster in a child with AIDS.Journal of neurovirology · 2019Article
- Herpes Zoster in the Older Adult.Infectious disease clinics of North America · 2017Review
- Differentiation between wild-type and vaccines strains of varicella zoster virus (VZV) based on four single nucleotide polymorphisms.Epidemiology and infection · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
29 authors at 13 institutions in 1 country.
Funding
Abstract
A real-time PCR assay was developed to identify varicella-zoster virus (VZV) and herpes simplex virus (HSV) DNA in clinical specimens from subjects with suspected herpes zoster (HZ; shingles). Three sets of primers and probes were used in separate PCR reactions to detect and discriminate among wild-type VZV (VZV-WT), Oka vaccine strain VZV (VZV-Oka), and HSV DNA, and the reaction for each virus DNA was multiplexed with primers and probe specific for the human beta-globin gene to assess specimen adequacy. Discrimination of all VZV-WT strains, including Japanese isolates and the Oka parent strain, from VZV-Oka was based upon a single nucleotide polymorphism at position 106262 in ORF 62, resulting in preferential amplification by the homologous primer pair. The assay was highly sensitive and specific for the target virus DNA, and no cross-reactions were detected with any other infectious agent. With the PCR assay as the gold standard, the sensitivity of virus culture was 53% for VZV and 77% for HSV. There was 92% agreement between the clinical diagnosis of HZ by the Clinical Evaluation Committee and the PCR assay results.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.