ArticleGene therapy2009
siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model.
Article in Gene therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- First-in-human mutation-targeted siRNA phase Ib trial of an inherited skin disorder.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Trial
- RNAi-based drug design: considerations and future directions.Nature reviews. Drug discovery · 2024Review
- Construction of histidine-containing hydrocarbon stapled cell penetrating peptides forChemical science · 2018Article
- Imaging of Tumor-Associated Macrophages in a Transgenic Mouse Model of Orthotopic Ovarian Cancer.Molecular imaging and biology · 2017Article
- Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling.Journal of biomedical science · 2016Article
- Non-Invasive Intravital Imaging of siRNA-Mediated Mutant Keratin Gene Repression in Skin.Molecular imaging and biology · 2016Article
- A small interfering RNA targeting vascular endothelial growth factor efficiently inhibits growth of VX2 cells and VX2 tumor model of hepatocellular carcinoma in rabbit by transarterial embolization-mediated siRNA delivery.Drug design, development and therapy · 2016Article
- Microneedles As a Delivery System for Gene Therapy.Frontiers in pharmacology · 2016Review
- Differential fates of biomolecules delivered to target cells via extracellular vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Guanidinium-rich, glycerol-derived oligocarbonates: a new class of cell-penetrating molecular transporters that complex, deliver, and release siRNA.Molecular pharmaceutics · 2015Article
- In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Gene Silencing in Skin After Deposition of Self-Delivery siRNA With a Motorized Microneedle Array Device.Molecular therapy. Nucleic acids · 2013Article
- Gene silencing following siRNA delivery to skin via coated steel microneedles: In vitro and in vivo proof-of-concept.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Multimodality imaging of RNA interference.Current medicinal chemistry · 2013Review
- Intravital fluorescence imaging of small interfering RNA-mediated gene repression in a dual reporter melanoma xenograft model.Nucleic acid therapeutics · 2012Article
- Designed guanidinium-rich amphipathic oligocarbonate molecular transporters complex, deliver and release siRNA in cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays.Human gene therapy · 2012Article
- Progress towards genetic and pharmacological therapies for keratin genodermatoses: current perspective and future promise.Experimental dermatology · 2012Review
- Progress toward in vivo use of siRNAs-II.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Review
- Disruption of cytokeratin-8 interaction with F508del-CFTR corrects its functional defect.Human molecular genetics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Small interfering RNAs (siRNAs) can be designed to specifically and potently target and silence a mutant allele, with little or no effect on the corresponding wild-type allele expression, presenting an opportunity for therapeutic intervention. Although several siRNAs have entered clinical trials, the development of siRNA therapeutics as a new drug class will require the development of improved delivery technologies. In this study, a reporter mouse model (transgenic click beetle luciferase/humanized monster green fluorescent protein) was developed to enable the study of siRNA delivery to skin; in this transgenic mouse, green fluorescent protein reporter gene expression is confined to the epidermis. Intradermal injection of siRNAs targeting the reporter gene resulted in marked reduction of green fluorescent protein expression in the localized treatment areas as measured by histology, real-time quantitative polymerase chain reaction and intravital imaging using a dual-axes confocal fluorescence microscope. These results indicate that this transgenic mouse skin model, coupled with in vivo imaging, will be useful for development of efficient and 'patient-friendly' siRNA delivery techniques and should facilitate the translation of siRNA-based therapeutics to the clinic for treatment of skin disorders.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.