ArticleInvestigative ophthalmology & visual science2009
Retinal endothelial cell apoptosis stimulates recruitment of endothelial progenitor cells.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Ghost vessels in the eye: Cell free choriocapillaris domains in atrophic age-related macular degeneration.Experimental eye research · 2024Article
- Tailoring of apoptotic bodies for diagnostic and therapeutic applications:advances, challenges, and prospects.Journal of translational medicine · 2024Review
- Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage.Neural regeneration research · 2020Article
- The Role of Inflammation in Diabetic Retinopathy.Frontiers in immunology · 2020Review
- Endothelial cell fitness dictates the source of regenerating liver vasculature.The Journal of experimental medicine · 2018Article
- Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing.International journal of molecular sciences · 2017Review
- The Role of the Lysyl Oxidases in Tissue Repair and Remodeling: A Concise Review.Tissue engineering and regenerative medicine · 2017Review
- Endothelial progenitor cells in diabetic retinopathy.Frontiers in endocrinology · 2014Review
- Molecular imaging of retinal disease.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2013Review
- Adiponectin-deficiency exaggerates sepsis-induced microvascular dysfunction in the mouse brain.Obesity (Silver Spring, Md.) · 2012Article
- Cell-based therapies for diabetic retinopathy.Current diabetes reports · 2011Review
- The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeBone marrow-derived endothelial progenitor cells (EPCs) contribute to vascular repair although it is uncertain how local endothelial cell apoptosis influences their reparative function. This study was conducted to determine how the presence of apoptotic bodies at sites of endothelial damage may influence participation of EPCs in retinal microvascular repair.
methodsMicrolesions of apoptotic cell death were created in monolayers of retinal microvascular endothelial cells (RMECs) by using the photodynamic drug verteporfin. The adhesion of early-EPCs to these lesions was studied before detachment of the apoptotic cells or after their removal from the wound site. Apoptotic bodies were fed to normal RMECs and mRNA levels for adhesion molecules were analyzed.
resultsEndothelial lesions where apoptotic bodies were left attached at the wound site showed a fivefold enhancement in EPC recruitment (P < 0.05) compared with lesions where the apoptotic cells had been removed. In intact RMEC monolayers exposed to apoptotic bodies, expression of ICAM, VCAM, and E-selectin was upregulated by 5- to 15-fold (P < 0.05-0.001). EPCs showed a characteristic chemotactic response (P < 0.05) to conditioned medium obtained from apoptotic bodies, whereas analysis of the medium showed significantly increased levels of VEGF, IL-8, IL-6, and TNF-alpha when compared to control medium; SDF-1 remained unchanged.
conclusionsThe data indicate that apoptotic bodies derived from retinal capillary endothelium mediate release of proangiogenic cytokines and chemokines and induce adhesion molecule expression in a manner that facilitates EPC recruitment.
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Registered trials
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