ArticleJournal of virology2009
The multiplicity of infection of a plant virus varies during colonization of its eukaryotic host.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 96 citations in OpenAlex.
- Identification of the Matrix Protein as a Conserved and Central Determinant of Superinfection Exclusion in Plant Rhabdoviruses.Molecular plant pathology · 2025Article
- Unveiling host-virus-vector interaction and latency phenomenon of banana bunchy top virus (BBTV) infecting banana.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq.The plant pathology journal · 2024Article
- Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis.Genome biology · 2021Article
- Plant death caused by inefficient induction of antiviral R-gene-mediated resistance may function as a suicidal population resistance mechanism.Communications biology · 2021Article
- Bottleneck, Isolate, Amplify, Select (BIAS) as a mechanistic framework for intracellular population dynamics of positive-sense RNA viruses.Virus evolution · 2020Review
- Modeling multipartite virus evolution: the genome formula facilitates rapid adaptation to heterogeneous environmentsVirus evolution · 2020Article
- The Matrix Protein of a Plant Rhabdovirus Mediates Superinfection Exclusion by Inhibiting Viral Transcription.Journal of virology · 2019Article
- Natural selection in compartmentalized environment with reshuffling.Journal of mathematical biology · 2019Article
- Multiscale modeling of influenza A virus replication in cell cultures predicts infection dynamics for highly different infection conditions.PLoS computational biology · 2019Article
- Variability Studies of TwoViruses · 2018Article
- Quantitative profiling of innate immune activation by viral infection in single cells.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Collective Infectious Units in Viruses.Trends in microbiology · 2017Review
- Antagonistic within-host interactions between plant viruses: molecular basis and impact on viral and host fitness.Molecular plant pathology · 2016Review
- Histone Deacetylase Inhibitors Reduce the Number of Herpes Simplex Virus-1 Genomes Initiating Expression in Individual Cells.Frontiers in microbiology · 2016Article
- Genetic variation in fitness within a clonal population of a plant RNA virus.Virus evolution · 2016Article
- Pathogen Propagation Model with Superinfection in Vegetatively Propagated Plants on Lattice Space.PloS one · 2016Article
- RNA Silencing May Play a Role in but Is Not the Only Determinant of the Multiplicity of Infection.Journal of virology · 2016Article
- A Population Genetics Perspective on Geminivirus Infection.Journal of virology · 2015Article
- The Multiplicity of Cellular Infection Changes Depending on the Route of Cell Infection in a Plant Virus.Journal of virology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multiplicity of infection (MOI), i.e., the number of virus genomes that infect a cell, is a key parameter in virus evolution, as it determines processes such as genetic exchange among genomes, selection intensity on viral genes, epistatic interactions, and the evolution of multipartite viruses. In fact, the MOI level is equivalent to the virus ploidy during genome expression. Nevertheless, there are few experimental estimates of MOI, particularly for viruses with eukaryotic hosts. Here we estimate the MOI of Tobacco mosaic virus (TMV) in its systemic host, Nicotiana benthamiana. The progress of infection of two TMV genotypes, differently tagged with the green or red fluorescent proteins GFP and RFP, was monitored by determining the number of leaf cell protoplasts that showed GFP, RFP, or GFP and RFP fluorescence at different times postinoculation. This approach allowed the quantitative analysis of the kinetics of infection and estimation of the generation time and the number of infection cycles required for leaf colonization. MOI levels were estimated from the frequency of cells infected by only TMV-GFP or TMV-RFP. The MOI was high, but it changed during the infection process, decreasing from an initial level of about 6 to a final one of 1 to 2, with most infection cycles occurring at the higher MOI levels. The decreasing MOI can be explained by mechanisms limiting superinfection and/or by genotype competition within double-infected cells, which was shown to occur in coinfected tobacco protoplasts. To our knowledge, this is the first estimate of MOI during virus colonization of a eukaryotic host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.