ArticleJournal of nanobiotechnology2009
QDs versus Alexa: reality of promising tools for immunocytochemistry.
Article in Journal of nanobiotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- NIR Excitation in Atomically Precise Nanoclusters via Two-Photon and Three-Photon Absorption.Accounts of chemical research · 2026Article
- Quantum Dot-Based Immunolabelling of Extracellular Vesicles and Detection Using Fluorescence-Based Nanoparticle Tracking Analysis.Journal of extracellular biology · 2025Article
- Review
- Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies.Biosensors · 2024Review
- Biosensors Based on Inorganic Composite Fluorescent Hydrogels.Nanomaterials (Basel, Switzerland) · 2023Review
- PLGA-Gold Nanocomposite: Preparation and Biomedical Applications.Pharmaceutics · 2022Review
- Luminescent nanomaterials for droplet tracking in a microfluidic trapping array.Analytical and bioanalytical chemistry · 2019Article
- Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets.Beilstein journal of nanotechnology · 2017Article
- Application of a biotin functionalized QD assay for determining available binding sites on electrospun nanofiber membrane.Journal of nanobiotechnology · 2011Article
- Observing GLUT4 translocation in live L6 cells using quantum dots.Sensors (Basel, Switzerland) · 2011Article
- Nucleoside conjugates of quantum dots for characterization of G protein-coupled receptors: strategies for immobilizing A2A adenosine receptor agonists.Journal of nanobiotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe unique photonic properties of the recently developed fluorescent semiconductor nanocrystals (QDs) have made them a potential tool in biological research. However, QDs are not yet a part of routine laboratory techniques. Double and triple immunocytochemistries were performed in HeLa cell cultures with commercial CdSe QDs conjugated to antibodies. The optical characteristics, due to which QDs can be used as immunolabels, were evaluated in terms of emission spectra, photostability and specificity.
resultsQDs were used as secondary and tertiary antibodies to detect beta-tubulin (microtubule network), GM130 (Golgi complex) and EEA1 (endosomal system). The data obtained were compared to homologous Alexa Fluor 594 organic dyes. It was found that QDs are excellent fluorochromes with higher intensity, narrower bandwidth values and higher photostability than Alexa dyes in an immunocytochemical process. In terms of specificity, QDs showed high specificity against GM130 and EEA1 primary antibodies, but poor specificity against beta-tubulin. Alexa dyes showed good specificity for all the targets tested.
conclusionThis study demonstrates the great potential of QDs, as they are shown to have superior properties to Alexa dyes. Although their specificity still needs to be improved in some cases, QDs conjugated to antibodies can be used instead of organic molecules in routine immunocytochemistry.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.