ArticleBlood2009
Differential expression of NADPH oxidases in megakaryocytes and their role in polyploidy.
Article in Blood, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Redox Regulation of Megakaryocyte Differentiation and Platelet Biogenesis.Antioxidants (Basel, Switzerland) · 2026Review
- Oxidative Stress and Mitochondrial Dysfunction in Myelodysplastic Syndrome: Roles in Development, Diagnosis, Prognosis, and Treatment.International journal of molecular sciences · 2025Review
- Neutrophil "plucking" on megakaryocytes drives platelet production and boosts cardiovascular disease.Immunity · 2022Article
- Replication of Dengue Virus in K562-Megakaryocytes Induces Suppression in the Accumulation of Reactive Oxygen Species.Frontiers in microbiology · 2021Article
- Reactive oxygen species in haematopoiesis: leukaemic cells take a walk on the wild side.Journal of experimental & clinical cancer research : CR · 2018Review
- NADPH oxidase in brain injury and neurodegenerative disorders.Molecular neurodegeneration · 2017Review
- NADPH oxidase-2 mediates zinc deficiency-induced oxidative stress and kidney damage.American journal of physiology. Cell physiology · 2017Article
- Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress.Oxidative medicine and cellular longevity · 2017Article
- Differential Roles of the NADPH-Oxidase 1 and 2 in Platelet Activation and Thrombosis.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- The role of Nox1 and Nox2 in GPVI-dependent platelet activation and thrombus formation.Redox biology · 2014Article
- ROS-mediated platelet generation: a microenvironment-dependent manner for megakaryocyte proliferation, differentiation, and maturation.Cell death & disease · 2013Review
- Megakaryocyte polyploidy is inhibited by lysyl oxidase propeptide.Cell cycle (Georgetown, Tex.) · 2013Article
- Oxidases and reactive oxygen species during hematopoiesis: a focus on megakaryocytes.Journal of cellular physiology · 2012Review
- Hemophagocytic macrophages in murine typhoid fever have an anti-inflammatory phenotype.Infection and immunity · 2012Article
- Developmental regulation of decidual cell polyploidy at the site of implantation.Frontiers in bioscience (Scholar edition) · 2012Review
- Hepatocytes polyploidization and cell cycle control in liver physiopathology.International journal of hepatology · 2012Article
- Control of megakaryocyte expansion and bone marrow fibrosis by lysyl oxidase.The Journal of biological chemistry · 2011Article
- Historical perspective and future directions in platelet research.Journal of thrombosis and haemostasis : JTH · 2011Review
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Megakaryocytes (MKs) undergo an endomitotic cell cycle, leading to polyploidy. We examined the expression of the flavoproteins and oxidative stress-promoting enzymes, NADPH oxidases (Nox's), in MKs because of their known role in promoting the cell cycle. Although the expression of Nox isoforms varies between cell types, they are induced at the mRNA level by mitogenic stimuli. Western blotting or reverse transcription-polymerase chain reaction of purified mouse MKs isolated from thrombopoietin (TPO)-treated bone marrow (BM) cultures indicated high expression of Nox1, a weak expression of Nox4, and no significant expression of Nox2. Immunofluorescence of freshly isolated MKs confirmed strong expression of Nox1 in one-third of MKs, whereas Nox1 staining was detected in nearly all MKs in TPO-stimulated BM cultures. Treatment of mouse BM cultures with Nox inhibitors resulted in accumulation of MKs with low DNA content levels and significant reduction of higher ploidy MKs. Purified, Nox-inhibited MKs showed a notable decrease in the level of the G(1) phase cyclin E, a cyclin associated with MK polyploidy, and its up-regulation restored most of the effect of Nox inhibitors. Hence, this study shows the expression of Nox isoforms in MKs and highlights a potential role of flavoproteins in promoting polyploidization in this lineage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.