ArticleThe Journal of physiology2009
Cyclic stretch reduces myofibrillar protein synthesis despite increases in FAK and anabolic signalling in L6 cells.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Dileucine-supplemented essential amino acids support whole-body anabolism after resistance exercise and serum-stimulated cell-based anabolism.Journal of the International Society of Sports Nutrition · 2025Trial
- Synchronous deficits in cumulative muscle protein synthesis and ribosomal biogenesis underlie age-related anabolic resistance to exercise in humans.The Journal of physiology · 2016Trial
- Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Trial
- Stable isotope-derived measurements of protein synthesis detect amino acid-induced anabolism in skeletal muscle microtissues not captured by puromycin labelling.Experimental physiology · 2026Article
- Biomechanical Stimulation of Muscle Constructs Influences Phenotype of Bone Constructs by Modulating Myokine Secretion.JBMR plus · 2023Article
- Effect of Mechanical Loading of Senescent Myoblasts on Their Myogenic Lineage Progression and Survival.Cells · 2022Article
- Inter-set stretch: A potential time-efficient strategy for enhancing skeletal muscle adaptations.Frontiers in sports and active living · 2022Review
- Blood Flow Restriction Training for the Intervention of Sarcopenia: Current Stage and Future Perspective.Frontiers in medicine · 2022Review
- Molecular and Biomechanical Adaptations to Mechanical Stretch in Cultured Myotubes.Frontiers in physiology · 2021Review
- A novel stable isotope tracer method to simultaneously quantify skeletal muscle protein synthesis and breakdown.Metabolism open · 2020Article
- Mechanical regulation of macrophage function - cyclic tensile force inhibits NLRP3 inflammasome-dependent IL-1β secretion in murine macrophages.Inflammation and regeneration · 2019Review
- FAK tyrosine phosphorylation is regulated by AMPK and controls metabolism in human skeletal muscle.Diabetologia · 2018Article
- Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies.Journal of the Royal Society, Interface · 2018Review
- Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity.Frontiers in veterinary science · 2018Article
- Molecular plasticity and functional enhancements of leg muscles in response to hypergravity in the fruit flyThe Journal of experimental biology · 2017Article
- Considerations on mTOR regulation at serine 2448: implications for muscle metabolism studies.Cellular and molecular life sciences : CMLS · 2017Review
- Effects of age and hindlimb immobilization and remobilization on fast troponin T precursor mRNA alternative splicing in rat gastrocnemius muscle.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2016Article
- Alcohol impairs skeletal muscle protein synthesis and mTOR signaling in a time-dependent manner following electrically stimulated muscle contraction.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.SpringerPlus · 2014Article
- CapZ and actin capping dynamics increase in myocytes after a bout of exercise and abates in hours after stimulation ends.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Muscle protein synthesis is increased after exercise, but evidence is now accruing that during muscular activity it is suppressed. In life, muscles are subjected to shortening forces due to contraction, but may also be subject to stretching forces during lengthening. It would be biologically inefficient if contraction and stretch have different effects on muscle protein turnover, but little is known about the metabolic effects of stretch. To investigate this, we assessed myofibrillar and sarcoplasmic protein synthesis (MPS, SPS, respectively) by incorporation of [1-13C]proline (using gas chromatography-mass spectrometry) and anabolic signalling (by phospho-immunoblotting and kinase assays) in cultured L6 skeletal muscle cells during 30 min of cyclic stretch and over 30 min intervals for up to 120 min afterwards. SPS was unaffected, whereas MPS was suppressed by 40 +/- 0.03% during stretch, before returning to basal rates by 90-20 min afterwards. Paradoxically, stretch stimulated anabolic signalling with peak values after 2-30 min: e.g. focal adhesion kinase (FAK Tyr576/577; +28 +/- 6%), protein kinase B activity (Akt; +113 +/- 31%), p70S6K1 (ribosomal S6 kinase Thr389; 25 +/- 5%), 4E binding protein 1 (4EBP1 Thr37/46; 14 +/- 3%), eukaryotic elongation factor 2 (eEF2 Thr56; -47 +/- 4%), extracellular regulated protein kinase 1/2 (ERK1/2 Tyr202/204; +65% +/- 9%), eukaryotic initiation factor 2alpha (eIF2alpha Ser51; -20 +/- 5%, P < 0.05) and eukaryotic initiation factor 4E (eIF4E Ser209; +33 +/- 10%, P < 0.05). After stretch, except for Akt activity, stimulatory phosphorylations were sustained: e.g. FAK (+26 +/- 11%) for > or =30 min, eEF2 for > or =60 min (peak -45 +/- 4%), 4EBP1 for > or =90 min (+33 +/- 5%), and p70S6K1 remained elevated throughout (peak +64 +/- 7%). Adenosine monophosphate-activated protein kinase (AMPK) phosphorylation was unchanged throughout. We report for the first time that acute cyclic stretch specifically suppresses MPS, despite increases in activity/phosphorylation of elements thought to increase anabolism.
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